食品与发酵工业2018，Vol.44Issue(4)：1-7,7.DOI:10.13995/j.cnki.11-1802/ts.016243
甜味剂莱鲍迪苷D的高效生物催化合成
Whole cell catalysis of sweetener rebaudioside D by recombinant E.coli
摘要
Abstract
An engineered E.coli strain was developed to produce the noncaloric sweetener rebaudioside D (RD) from rebaudioside A (RA) through whole cell biocatalysis.In order to increase endogenous production of the essential active sugar donor of the catalytic reaction,uridine diphosphate glucose (UDPG),host strain E.coli BL21 (DE3) was subjected to chromosomal modifications and the resultant genetically modified host strains were named as SG1,SG2,SG3 and SG4,respectively.These modified hosts were transformed with plasmid pYF09 harboring the glycosyl-transferase EUGT11 that catalyze the biosynthesis of RD from RA,and the resultant engineered cells were then used to catalyze the biosynthesis of RD.The strain pYF09-SG4 was selected for further studies on whole cell catalysis conditions.The results showed that using fresh cells collected from 50 mL liquid culture and under conditions 100mmol/L pH 8.0 sodium phosphate buffer 5 mmol/L ZnCl2,80 mmol/L sodium citrate,0.1％ volumetric percent of Triton X100,500 g/L sucrose,temperature 42 ℃,and 1 d incubation time,98.5％ of 1 mmol/L RA was catalyzed to RD with a yield of 1 112.21 mg/L.关键词
甜叶菊/莱鲍迪苷A(Rebaudioside A,RA)/莱鲍迪苷D(Rebaudioside D,RD)/重组大肠杆菌/全细胞催化Key words
Stevia rebaudiana/rebaudioside A (RA)/rebaudioside D (RD)/recombinant E.coli/whole-cell catalysis引用本文复制引用
费理文,王勇..甜味剂莱鲍迪苷D的高效生物催化合成[J].食品与发酵工业,2018,44(4):1-7,7.基金项目
国家自然科学基金(31670099) （31670099）
中国科学院STS项目(KFJ-SW-STS-164-08) （KFJ-SW-STS-164-08）
