食品与发酵工业2018，Vol.44Issue(4)：60-64,5.DOI:10.13995/j.cnki.11-1802/ts.015455
莠去津降解菌的筛选及其降解特性研究
Screening and characterization of atrazine-degrading strains
摘要
Abstract
The atrazine-degrading strains were screened using solid culture with high concentration of atrazine and high performance liquid chromatography (HPLC).The dynamics of growth and atrazine degradation rate were determined,and the effects of co-substrates and environmental condition on degradation rate were also evaluated.The results showed that 10 strains had degradative capability for atrazine with the degradation rate from 13.3％ to 41.1％,and the maximum degradation rate was obtained with strain XQB-33.Among 10 strains,4 strains (XQB-1,XQB-21,XQB-25,XQB-33) showed high atrazine degradation capacity with degradation rate of 38.1％,34.6％,37.3％ and 41.1％,respectively.Furthermore,theses 4 strains reached logarithmic phase and showed the highest atrazine degradation rate after culture in liquid medium for 72 h.After addition of glucose,sodium citrate,sucrose and succinic acid sodium acetate as co-substrates,the degradation rate of 4 strains increased significantly,especially for addition of 0.5％ glucose,the degradation rate of XQB-1 and XQB-1 increased by 15.2％ and 14.3％,respectively.The environmental factors can affect the degradation efficiency.The optimum degradation conditions for these 4 strains were as follows:inoculation amount of 2.0％,initial atrazine concentration of 100-200 mg/L,initial pH value of 5-6,culture temperature of 30 ℃,and volume of 75-100 mL/150 mL.关键词
莠去津降解菌/生长动态/降解率/共代谢物/环境条件Key words
atrazine-degrading strains/dynamics of growth/degradation rate/co-substrates/environmental condition引用本文复制引用
冯瑞章,杜永华,魏琴,周万海,何飞,黄彭,李莉..莠去津降解菌的筛选及其降解特性研究[J].食品与发酵工业,2018,44(4):60-64,5.基金项目
四川省教育厅项目(13ZA0201) （13ZA0201）
发酵资源与应用四川省高校重点实验室项目(2010KFZ004,2011KFJ003) （2010KFZ004,2011KFJ003）
固态发酵资源利用四川省重点实验室项目(2015GTY006,2016GTY002) （2015GTY006,2016GTY002）
