食品与发酵工业2018，Vol.44Issue(4)：234-240,7.DOI:10.13995/j.cnki.11-1802/ts.014678
小麦和榛子过敏原成分检测的实时荧光PCR方法
Real-time fluorescence PCR in allergens detecting of wheat and hazelnuts
摘要
Abstract
Food allergies are in increasing issue in food safety.Therefore,the evaluation and detection of food allergens become more and more important.To quickly and accurately detect food allergens is an urgent need.At present,the most common method used in allergen detection are immunological detection,mass spectrometry and SPR technology.However,there are some limitations in these methods.We used molecular biology techniques and established a database of allergen genes for wheat and hazelnut,and designed primers by finding the specific sequences.In order to find a rapid,convenient and efficient method for the detection of allergen,we implemented a real time fluorescence PCR method for a rapid screening of the allergen gene.It is simple,low-cost,easy for operation and time saving.The detection limit was increased,and miss-dection rate was lowed.Through the applicability test and LOD experiments,we found the method is applicable for the identification of allergens,good repeatability and accuracy with the detecting limit of 1％.关键词
过敏原成分检测/实时荧光PCR/检出限Key words
allergen detection/real-time fluorescence PCR/limit of determination (LOD)引用本文复制引用
尚柯,张彪,段庆梓,张玉,王巍,梁恒兴..小麦和榛子过敏原成分检测的实时荧光PCR方法[J].食品与发酵工业,2018,44(4):234-240,7.基金项目
成都市科技惠民应用示范项目(2015-HM02-00099-SF) （2015-HM02-00099-SF）
