食品科学技术学报2018，Vol.36Issue(3)：25-32,55,9.DOI:10.3969/j.issn.2095-6002.2018.03.004
我国市售食用菌中金黄色葡萄球菌污染调查、耐药性及其肠毒素基因检测
Prevalence, Antibiotic Resistance, and Enterotoxin Gene Detection of Staphylococcus aureus Isolated from Retail Edible Mushrooms in China
摘要
Abstract
This study investigated the contamination level of Staphylococcus aureus of retail edible mush-rooms in China. 699 retail edible mushroom samples were collected from 39 cities in China during 2011 to 2016,33 samples(4.72%) were positive for Staphylococcus aureus and the geometric mean was 9.75 MPN/g. A total of 39 isolates were analyzed.For antibiotics susceptibility tests, all isolated strains were resistant to 22 kinds of different antibiotics,such as ampicillin,and only sensitive to linezolid and rifam-picin. Seven isolates were resistant to cefoxitin and carried mecA gene,confirmed as methicililin-resistant Staphylococcus aureus (MRSA).Polymerase chain reaction(PCR) analysis of 18 SE genes showed that 4-6 kinds of enterotoxin genes carried by 60% isolated strains,and sei gene was the most frequently de-tected,followed by sem,seg,seq,sec,seb,sen.This study reflects the potential risk of Staphylococcus au-reus contamination of retail edible mushrooms in China and these isolate strains had virulence potential and drug tolerance,which should be drawn public attention.关键词
金黄色葡萄球菌/食用菌/污染水平/肠毒素基因/耐药性Key words
Staphylococcus aureus/edible mushrooms/pollution level/enterotoxin gene/drug tolerance分类
轻工纺织引用本文复制引用
黄嘉慧,吴诗,张峰,张菊梅,吴清平..我国市售食用菌中金黄色葡萄球菌污染调查、耐药性及其肠毒素基因检测[J].食品科学技术学报,2018,36(3):25-32,55,9.基金项目
中国博士后科学基金资助项目(2017M612623) （2017M612623）
广州市科技计划项目(201504010036,201508020068) （201504010036,201508020068）
广东省科学院创新驱动发展能力专项(2017GDASCX-0201,2017GDASCX-0817). （2017GDASCX-0201,2017GDASCX-0817）
