食品科学技术学报2018，Vol.36Issue(3)：40-47,8.DOI:10.3969/j.issn.2095-6002.2018.03.006
天麻醇提物对灰树花胞外多糖单糖组成和生物活性的影响
Effects of Rhizoma gastrodiae Alcohol Extract on Monosaccharide Composition and Biological Activity of Grifola frondosa Exopolysaccharide
摘要
Abstract
The composition and anti-tumor bioactivity of the exopolysaccharide monosaccharide was car-ried out by adding Rhizoma gastrodiae alcohol extract to the liquid fermentation system of Grifola frondosa. The HPLC was applied to analyse the monosaccharide composition of exopolysaccharide samples and re-sults showed that the crude exopolysaccharide samples were composed of glucose,mannose,and a small amount of galactose. Whereas the mannose, rhamnose, glucose, galactose, arabinose, fucose etc were the main monosaccharide composition of pure exopolysaccharides. Furthermore, the addition of Rhizoma gastrodiae alcohol extract had a significant change in the content of the monosaccharide component of the exopolysaccharide,but did not produce the new monosaccharide component of the polysaccharide. In the sample of pure exopolysaccharides,the monosaccharide molar ratio of mannose:rhamnose:glucose:ga-lactose,arabinose:fucose was 13.8:3.9:5:3.7:1:1.7, while the pure exopolysaccharides fermented with Rhizoma gastrodiae monosaccharide mole ratio was 12.7:3.2:5.6:3.5:1:1.6. The results about of the biological activity Grifola frondosa exopolysaccharide on mice macrophage(RAW264.7) showed that the pure exopolysaccharides fermented with Rhizoma gastrodiae had obvious activation on macrophage. The pure exopolysaccharides and exopolysaccharides obtained by fermentation with Rhizoma gastrodiae had certain activation on macrophage. The activation of macrophages by crude exopolysaccha-rides was not significant. In vitro, four polysaccharide samples showed no inhibitory effect on human hepatoma HepG 2 cells.关键词
天麻醇提物/灰树花/单糖组成/小鼠巨噬细胞RAW264.7/人肝癌细胞HepG2Key words
Rhizoma gastrodiae alcohol extract/Grifola frondosa/monosaccharide composition/mice macrophage RAW264.7/human hepatoma HepG 2 cells分类
轻工纺织引用本文复制引用
芦红云,吴天祥,汤庆莉,钟敏,聂文强..天麻醇提物对灰树花胞外多糖单糖组成和生物活性的影响[J].食品科学技术学报,2018,36(3):40-47,8.基金项目
国家自然科学基金地区科学基金项目(31460537). （31460537）
