中国食品添加剂Issue(4)：99-105,7.
复合酶法提取黄秋葵多糖的响应面优化研究
Optimization of enzymatic extraction of okra polysaccharides by response surface methodology
摘要
Abstract
To optimize enzymatic extraction process of polysaccharide from okra,experiments of single factor were conducted.Then under the condition of solid-liquid ratio of 1 ∶ 30,pH5.5,the ratio of pectinase to cellulase 1 ∶3,3 factors and 3 levels Box-Behnken central composite test was designed to study the effect of compound enzyme amount,extraction temperature and extraction time on the extraction rate of okra polysaccharide.The optimum technological parameters of enzymatic extraction of okra polysaccharide were:extraction time 73min,extraction temperature 62 ℃ and compound enzyme amount 0.56％.Under the above condition the predictive extraction rate of polysaccharides is 6.72％.The actual testing value is 6.41％,and the relative deviation from the predicted maximum response value is 4.61％.关键词
黄秋葵/多糖/复合酶法/响应面分析Key words
okra/polysaccharide/compound enzymatic extraction/response surface analysis optimization分类
轻工纺织引用本文复制引用
雍成文,王文玲,李湘球,赵志刚,韩成云..复合酶法提取黄秋葵多糖的响应面优化研究[J].中国食品添加剂,2018,(4):99-105,7.基金项目
江西省“2011协同创新中心”(天然药物活性成分研发与应用) （天然药物活性成分研发与应用）
宜春学院地方发展中心项目(黄秋葵嫩果促生长作用物质的提取及应用研究) （黄秋葵嫩果促生长作用物质的提取及应用研究）
萍乡市科技研发计划项目(黄秋葵种质资源创制与综合开发利用). （黄秋葵种质资源创制与综合开发利用）
