乳业科学与技术2018，Vol.41Issue(2)：22-29,8.DOI:10.15922/j.cnki.jdst.2018.02.005
类植物乳杆菌L-ZS9培养基及培养条件的优化
Optimization of Medium Composition and Culture Conditions for Lactobacillus paraplantarum L-ZS9
摘要
Abstract
Lactobacillus paraplantarum L-ZS9, isolated from fermented meat foods, as a probiotic strain and a fermentation starter has a great application potential in the dairy industry. In order to achieve high-density culture of strain L-ZS9, the modified MRS medium and culture conditions were optimized. The optimal carbon and nitrogen sources were selected by one-factor-at-a-time method. The Plackett-Burman experimental design was used to recognize the most significant medium components and culture conditions. The steepest ascent method was used to determine the levels for these variables. A central composite rotary design (CCRD) was used to fit cell density as a function of the independent variables and response surface methodology was employed to determine optimal culture conditions. The results showed that sucrose and yeast powder were used as carbon and nitrogen sources, respectively. The optimized medium formulation consisted of sucrose 3％, yeast extract 7.26％, sodium acetate 0.5％, dipotassium phosphate 0.2％, magnesium sulfate 0.02％, triammonium citrate 0.2％, and Tween-800.1％; the optimized culture conditions were as follows: the initial pH value of the medium was 9.53, the volume of inoculum was 1.74％, and the culture temperature was 37℃. It was verified that after 14 hours of cultivation under the optimized conditions, the optical density at wavelength of 600 nm of the broth was 1.214, and the viable count was 4.53× 109 CFU/mL at this time, which was 6 times higher than that before optimization.关键词
类植物乳杆菌/高密度培养/响应面优化Key words
Lactobacillus paraplantarum/high density culture/response surface methodology分类
轻工纺织引用本文复制引用
王世博,王丹婷,张金兰,李平兰..类植物乳杆菌L-ZS9培养基及培养条件的优化[J].乳业科学与技术,2018,41(2):22-29,8.基金项目
国家自然科学基金面上项目(31671831) （31671831）
北京市学生创新研究专项(2017JW001) （2017JW001）
