食品工业科技2018，Vol.39Issue(12)：16-20,5.DOI:10.13386/j.issn1002-0306.2018.12.004
双孢菇菇柄多糖柱层析纯化及单糖组成
Column chromatographic purification and monosaccharide composition of polysaccharide extracted from Agaricus bisporus stipe
摘要
Abstract
Using Agaricus bisporus stipe as material,firstly,the A.bisporus stipe polysaccharide was extracted by ultrasonic extraction,and purified using column chromatography.Then the purity of polysaccharides isolated by column chromatography was examined by UV spectrum and column chromatography.The infrared spectrum and monosaccharide composition of the isolated polysaccharides were also analyzed.Results showed that the extracted polysaccharide was divided into 4 components by DEAE Sephadex A-25 purification,and the components eluted by distilled water and 0.1 mol/L NaCl were collected.UV spectrum scan showed that there were no nucleic acids and proteins in both the elutes,and they were also showed a single peak using Sephadex G-200.Infrared spectrum showed the both components contained characteristic absorption peaks of polysaccharide,and they wereβ-pyranose polysaccharides.The polysaccharide eluted by distilled water was composed by glucose,galactose,mannose,xylose and arabinose.And the 0.1 mol/L NaCl eluting component consists of glucose,galactose,mannose,xylose and arabinose,rhamnose,glucosamine and galactosamine.关键词
双孢菇菇柄/多糖/柱层析纯化/红外光谱/单糖组成Key words
Agaricus bisporus stipe/polysaccharide/column chromatographic purification/infrared spectrum/monosaccharide composition分类
轻工纺织引用本文复制引用
李顺峰,王安建,田广瑞,刘丽娜,魏书信..双孢菇菇柄多糖柱层析纯化及单糖组成[J].食品工业科技,2018,39(12):16-20,5.基金项目
河南省农业科学院科研发展专项资金项目(YCY20167810). （YCY20167810）
