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泡菜中对铅有高吸附性的乳酸菌的分离鉴定及特性研究

代启虎 欧杰 李冉 李柏林

食品工业科技2018,Vol.39Issue(12):108-113,6.
食品工业科技2018,Vol.39Issue(12):108-113,6.DOI:10.13386/j.issn1002-0306.2018.12.020

泡菜中对铅有高吸附性的乳酸菌的分离鉴定及特性研究

Isolation,identification and characteriation of lactic acid bacteria with high adsorption to lead in pickles

代启虎 1欧杰 2李冉 1李柏林3

作者信息

  • 1. 上海海洋大学食品学院,上海201306
  • 2. 农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306
  • 3. 上海水产品加工及贮藏工程技术研究中心,上海201306
  • 折叠

摘要

Abstract

Lead-resistant and adsorptivity lactic acid bacteria were screened out from pickles and used for the removal of heavy metal lead.Four strains were isolated from the samples by spread plate method,and lead resistant reached 2000 mg/L,when the concentration of lead was 100 mg/L,,the adsorption rate of p3 was highest,reached 78.90%.The results of morphology observation,physiological and biochemical identification and mass spectrometry detection and 16S ribosomal deoxyribonucleic acid sequencing data showed that p3 could be identified as Lactobacillus brevis.The strain grew well in the salt containing 0~5%,and it was able to tolerate the hydrochloric acid with pH value of 2,the salts of 8.0 g/L and trypsin of 1.1 g/L.It can be used to treat waste water,make pickles orreduce lead levels in animals as a probiotic in the future.

关键词

泡菜//乳酸菌/分离与鉴定/特性

Key words

pickles/lead/lactic acid bacteria/isolation and identification/characteristic

分类

轻工纺织

引用本文复制引用

代启虎,欧杰,李冉,李柏林..泡菜中对铅有高吸附性的乳酸菌的分离鉴定及特性研究[J].食品工业科技,2018,39(12):108-113,6.

基金项目

国家自然科学基金(31671779). (31671779)

食品工业科技

OA北大核心CSTPCD

1002-0306

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