食品工业科技2018，Vol.39Issue(12)：119-123,131,6.DOI:10.13386/j.issn1002-0306.2018.12.022
产β-D-葡萄糖苷酶乳酸菌的筛选、鉴定及系统发育分析
Screening,identification and phylogenetic analysis of Lactic acid bacteria producing β-D-glucosidase
摘要
Abstract
In search for a glycosidase that can enhance flavor in fruit wine.Four strains of β-D-glucosidase were screened from 63 strains of Lactobacillus with Esculin color-developing plate method.The p-nitro-phenyl-β-D-glucopyranoside (PNPG) were used as a substrate and method of differential centrifugation graded precipitation was utilized to the initial location of β-D-glucosidase.In addition,traditional identification methods,such as morphology,physiological and biochemical tests,and sequence analysis of 16S rDNA were used to identify the four strains.The result indicated that the β-D-glucosidases generated by four strains were all endoenzyme;FL12 and Hsb were Lactobacillus plantarum,and CM3 was Lactobacillus pentosus and T61 was Lactobacillus brevis.关键词
乳酸菌/β-D-葡萄糖苷酶/鉴定/16S rDNA序列分析/系统发育树Key words
Lactic acid bacteria/β-D-glucosidase/identification/16S rDNA sequence analysis/phylogenetic tree分类
轻工纺织引用本文复制引用
周俊,夏秀东,李亚辉,王英,董明盛,周剑忠..产β-D-葡萄糖苷酶乳酸菌的筛选、鉴定及系统发育分析[J].食品工业科技,2018,39(12):119-123,131,6.基金项目
江苏省农业科技自主创新项目(CX(15)1026). （CX(15）
