食品工业科技2018，Vol.39Issue(12)：137-141,170,6.DOI:10.13386/j.issn1002-0306.2018.12.025
福建海参内源微生物多样性分析及益生菌抑菌机理初探
Biodiversity analysis and pathogen inhibition mechanism of the endogenous bacteria in Fujian Apostichopus japonicus
摘要
Abstract
For the purpose of exploring the microbial community structure and bacteriostasis mechanism of Fujian cage farming Apostichopus japonicus,diversity of the dominant strains isolated from the intestinal tracts of Apostichopus japonicus was analyzed using 16S rDNA sequence homology comparison method.The bacteriostatic ability of probiotics and their metabolites lipopeptide compounds were determined,and their antibacterial components were preliminarily determined by thin layer chromatography (TLC)..The results showed that 95 strains of dominant bacteria were isolated and purified,which included 31 strains of potential probiotics,37 strains of potential pathogens and 27 strains of common intestinal bacteria.Microbial community structure analysis found that the abundance of Pseudoalteromonas and Acinetobacter genera were higher while that of Bacillus genus was lower in diseased group than those in healthy group.It would be one of the possible causes of the body surface lesion of Fujian Apostichopusjaponicus.Taking 8 strains of typical pathogenic strains as indicator bacteria,two probiotic strains with wide antimicrobial spectrum and high bacteriostatic ability were screened out,which were Bacillus amyloliquefaciens YD1 and Bacillus subtilis YK5.Their lipopeptide metabolites had obvious bacteriostatic effect.The bacteriostasis components were preliminarily determined to be cyclic peptides containing hydrophilic components.The study provided an important basis for the development of functional probiotics and novel microecologics.关键词
海参/群落结构/脂肽类化合物/抑菌机理Key words
Apostichopus japonicus/microbial community structure/lipopeptide/antibacterial mechanism分类
轻工纺织引用本文复制引用
李智,黄晓山,郑宝东,邓凯波..福建海参内源微生物多样性分析及益生菌抑菌机理初探[J].食品工业科技,2018,39(12):137-141,170,6.基金项目
福建省教育厅科技项目(JB13050) （JB13050）
福建农林大学高水平建设项目(612014042) （612014042）
福建省高等学校科技创新团队支持计划(闽教科[2012]033号) （闽教科[2012]033号）
福建农林大学科技创新团队支持计划(cxtd12009). （cxtd12009）
