食品工业科技2018，Vol.39Issue(12)：154-163,10.DOI:10.13386/j.issn1002-0306.2018.12.028
超声-微波协同提取杜仲树皮及树叶中的黄酮类化合物
Extractions of the flavonoids from barks and leaves of Eucommia ulmoides Oliver by simultaneous ultrasonic/microwave assisted extraction
摘要
Abstract
To improve the extraction yields of flavonoids from Eucommia ulmoides Oliver by simultaneous ultrasonic/microwave assisted extraction(UMAE)method.The degummed E.ulmoides bark and leaf powders were taken as raw materials.The single factor experiment and response surface analysis were used to optimize the extraction conditions by UMAE.Furthermore,the flavonoids in the barks and leaves of E.ulmoides were also compared using HPLC combined standards.The results showed that the optimal conditions were as follows:ethanol concentration,the ratio of plant material to liquid,extraction temperature,microwave power and extraction time were 58％,70∶1 (mL∶ g),75 ℃,420 W and 6 min.Under these optimal conditions,the yield of flavonoids was 11.80％ ± 0.09％.Meanwhile,the present simultaneous extraction method was compared with microwave assisted extraction(MAE) and ultrasonic assisted extraction (UAE),and the mechanism of the present extraction method was also discussed.By comparison,the extraction yield of flavonoids from E.ulmoides by UMAE was significantly higher than MAEand UAE(p ＜0.01),which also showed higher DPPH free radical scavenging ability(p ＜ 0.01).The results from scanning electron microscope(SEM) indicated that the extraction efficiency of UMAE was higher,since the superiority of UMAE was simultaneously acted and mutually reinforced by ultrasonic and microwave.Consequently,UMAE could crush the plant cells more thoroughly than MAE and UAE,leading to more sufficient extraction,and thus the extraction rate and efficiency were significantly improved.Furthermore,it was found that flavonoids such as rutin,quercetin and kaempferol were significantly higher in leaves than barks,revealing E.ulmoides leaves could be used as better extraction materials for flavonoids than barks.关键词
杜仲黄酮/超声-微波协同提取(UMAE)/响应面分析/扫描电镜Key words
flavonoids from Eucommia ulmoides Oliver/ultrasonic/microwave assisted extraction (UMAE)/response surface analysis/scanning electron microscope(SEM)分类
轻工纺织引用本文复制引用
王颖,李荣,姜子涛,闫长旭,李婷婷,梁露露,何海军..超声-微波协同提取杜仲树皮及树叶中的黄酮类化合物[J].食品工业科技,2018,39(12):154-163,10.基金项目
天津市自然科学基金资助项目(16JCYBJC43300) （16JCYBJC43300）
国家大学生创新创业训练计划项目(201710069027). （201710069027）
