食品工业科技2018，Vol.39Issue(12)：171-176,6.DOI:10.13386/j.issn1002-0306.2018.12.030
桦褐孔菌黄酮类化合物的提取工艺优化及抗氧化活性
Optimization of extracted process of flavonoids from Inonotus obliquus and evaluation of antioxidant activity
摘要
Abstract
Taking Inonotus obliquus as test material,the single factor(solid-liquid ratio,extraction temperature,concentration of ethanol,extraction time)on extraction rate of flavonoids from Inonotus obliquus were studied,and the extraction methods were optimized by orthogonal test.Antioxidant activity of samples was determined from three aspects,using the method of FRAP,DPPH ·,ABTS + ·.The results showed that the effect of flavone content from Inonotus obliquus were in order extraction temperature ＞ concentration of ethanol ＞ extraction time ＞ solid-liquid ratio.The optimum extraction conditions were as follows:The extraction temperature was 75 ℃,the concentration of ethanol was 60％,the extraction time was 2.0 h,the ratio of material to liquid was 1 ∶ 25 g/mL,the content of flavonoids was 53.25 mg/mL,the extraction rate was 10.66％.When the concentration of flavonoids of Inonotus obliquus was 200 μg/mL,the total antioxidant capacity of that was equivalent to 464.53 μ mol/L FeSO4.The EC50 of clearance on DPPH· was 36.44 μg/mL,the EC50 of clearance on ABTS+ · was 299.89 μg/mL.The results showed that the clearance rate of flavonoids from Inonotus obliquus for DPPH· and ABTS + · was close to Vc,which proved strong antioxidant activity and potential natural antioxidants used in the future.关键词
桦褐孔菌/黄酮/抗氧化活性Key words
Inonotus obliquus/flavonoids/antioxidant activity分类
轻工纺织引用本文复制引用
张静,孙晶波,盛瑜,安丽萍,杜培革,郭笑..桦褐孔菌黄酮类化合物的提取工艺优化及抗氧化活性[J].食品工业科技,2018,39(12):171-176,6.基金项目
长白山药用动植物活性多肽研究与开发国家地方联合工程实验室资助项目(发改高技[2010]2455号) （发改高技[2010]2455号）
吉林省科技发展计划项目(20170520033JH) （20170520033JH）
吉林市科技创新发展计划项目(20165014). （20165014）
