食品工业科技2018，Vol.39Issue(12)：261-266,6.DOI:10.13386/j.issn1002-0306.2018.12.046
高效液相色谱-串联质谱法检测副溶血弧菌生物被膜形成过程中的N-酰基高丝氨酸内酯信号分子
Detecting of quorum sensing signal molecules N-acyl-homoserine lactones during the biofilm formation of V.parahaemolyticus by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)
摘要
Abstract
N-acyl-homoserine lactones (AHLs)are typical quorum sensing signal molecules and regulate the expression of many physiological characteristic in V.parahaemolyticus.At present,there is a lack of accurate detection methods for AHLs in biofilms.Based on the existing conditions of HPLC-MS/MS,detection methods for AHLs could be optimized during the biofilm formation of V.parahaemolyticus.It was identified that absolute methanol:0.1％ ammonium acetate water as the mobile phase separation could help to obtain better results.If the collision energy was 10～ 15 eV,a higher proportion of characteristic fragment ions(m/z) 102.1 and 74.1 could be formed for each signal molecule.Good linear relationship was obtained in the wide range of 0～20 μg/L(R2 ＞0.995).This method could help to detect three different signal molecules,including C4-HSL,3-oxo-C6-HSL and 3-oxo-C14-HSL,which could be formed in the formation process of biofilms of V.parahaemolyticus.Among these signal molecules,the amount of C4-HSL was higher than others(＞91.67％).In the early stage of biofilm formation(0.5～3 d),the concentration of AHLs increased slowly and was positively relate to the biofilm formation.In the discursive stage(4～5 d),the concentration of signa molecules increased rapidly.Furthermore,a negative correlation could be found significouldtly between the concentration of signal molecules and biofilm formation.The optimization of this method could help to effectively detect the AHLs of biofilm and provided support for further study of regulative relations between biofilm and the quorumsensing as well.关键词
高效液相色谱串联质谱法/N-酰基高丝氨酸内脂/生物被膜Key words
high performance liquid chromatography-tandem mass spectrometry/N-acyl lactone/biofilm分类
轻工纺织引用本文复制引用
郭沐晗,廖建萌,孙力军,王雅玲,房志家,刘颖,徐德峰..高效液相色谱-串联质谱法检测副溶血弧菌生物被膜形成过程中的N-酰基高丝氨酸内酯信号分子[J].食品工业科技,2018,39(12):261-266,6.基金项目
国家基金面上项目(31371746) （31371746）
广东省高等学校创新强校工程计划(GDOU2013050205,GDOU20014050203). （GDOU2013050205,GDOU20014050203）
