食品工业科技2018，Vol.39Issue(12)：271-274,300,5.DOI:10.13386/j.issn1002-0306.2018.12.048
超低温冷冻除脂-气相色谱串联质谱法检测食用植物油中21种邻苯二甲酸酯
Determination of 21 phthalic acid esters in edible vegetable oils by gas chromatography-tandem mass spectrometry with removing fat through ultra low temperature freezing
摘要
Abstract
A new method was developed for the determination of 21 Phthalic Acid Esters (PAEs)in edible vegetable oils by gas chromatography tandem mass spectrometry (GC-MS/MS)with removing fat through ultra low temperature freezing.With acetonitrile as extractant,sample was extracted and frozen with ultra low temperature at-80 ℃ for 10 min to remove fat,and then the sample solution was evaporated to near dryness by rotary evaporator and redissolved by n-hexane,and then detected by GC-MS/MS under multiple reaction monitoring modes.All analytes had good linearity with correlation coefficient over 0.999 in the investigated range(1～1000 ng/mL),the limits of detection(LOD) were 0.10～8.52 μg/kg,the limits of quantitation (LOQ)were 0.32～28.40 μg/kg,the recoveries were 74.31％ ～116.62％ with the relative standard deviation ranged of 1.21 ％ ～17.82％.Seven categories of 19 kinds of vegetable oil were examined,the results indicated that dimethyl phthalate(DMP),diethyl phthalate (DEP),diisobutylphthalate (DIBP),di-n-butyl phthalate (DBP),butyl benzyl phthalate (BBP),bis (2-butoxyethyl) phthalate (DBEP),di-n-heptyl phthalate (DHP),bis (2-ethylhexyl) phthalate (DEHP) and di-n-octyl phthalate(DNOP) total of 9 PAEs were detected,including DMP,DBP,DBEP,DHP and DEHP detection rate of more than 90％ with the content in the range of 105.57～2156.76 μg/kg.The method was easy to operate and low cost,which could be used for the effective determination of PAEs in edible vegetable oil.关键词
气相色谱串联质谱/邻苯二甲酸酯/超低温冷冻除脂/食用植物油Key words
gas chromatography-tandem mass spectrometry/phthalic acid esters/removing fat through ultra low temperature freezing/edible vegetable oil分类
轻工纺织引用本文复制引用
马蒙蒙,王宗义,贾明宏,王国庆,盛晔琪,翟孟婷,郑宇..超低温冷冻除脂-气相色谱串联质谱法检测食用植物油中21种邻苯二甲酸酯[J].食品工业科技,2018,39(12):271-274,300,5.基金项目
北京市教委项目(PXM2017-014207-000038) （PXM2017-014207-000038）
北京农学院实验技术体系基金项目(1086716287). （1086716287）
