食品与机械2018，Vol.34Issue(1)：130-134,5.DOI:10.13652/j.issn.1003-5788.2018.01.026
牡荆素保护DNA氧化损伤的活性评价及机制分析
Active evaluation of DNA protection to oxidative damage and its possible mechanisms of vitexin in vitro
摘要
Abstract
In order to study the DNA protection and possible mechanisms of vitexin,it was measured by using three spectrophotometric assays,including DNA protection,Fe2+-complexing,and Cu2+ reducing.In addition,its Fe2+-complexing reaction was also analyzed using UV-Vis spectra.The results showed that the IC50 values of vitexin were (919.6±23.6),(9.3±1.2) and (421.7±29.2) μmol/L,respectively,for DNA protection assay,Fe2+-complexing assay,and Cu2+ reducing assay.When mixed with Fe2+,vitexin gave a UV-Vis peak at 554 nm [molar absorptivity ε =2 680.74 L/(mol · cm)].In conclusion,vitexin can effectively protect DNA from · OH induced damage.The protective mechanism may include electron-transfer (ET) and Fe2+ complexing,and its Fe2+-complexing site is possibly between 4-carbonly and 5-hydroxyl groups.关键词
牡荆素/DNA氧化损伤/抗氧化/电子转移Key words
vitexin/oxidative DNA damage/antioxidation/electron transfer引用本文复制引用
陈班,李达欢,李熙灿..牡荆素保护DNA氧化损伤的活性评价及机制分析[J].食品与机械,2018,34(1):130-134,5.基金项目
国家自然科学基金项目(编号:81573558) （编号:81573558）
粤港联合创新项目(编号:2017A050506043) （编号:2017A050506043）
