食品与机械2018，Vol.34Issue(1)：135-139,172,6.DOI:10.13652/j.issn.1003-5788.2018.01.027
五味子油超声辅助提取工艺优化及其护肝作用
Study on the ultrasonic-assisted extraction process of Fructus Schizandrae oil and the hepatic protection effect
摘要
Abstract
Using the extraction yield of Fructus Schisandrae seed oil as an index,the ultrasonic extraction process of it was optimized by single factor and orthogonal design.HPLC was used to analyze the content of schizandrin A,B,and schisandrin in the seeds.The mice liver injuried model was established by administration with CCL4 and the contents of GSH,MDA and SOD in mice liver tissue were determined.HE staining was used to observe the pathological changes of liver.The results showed that the optimum process was as follows:the extraction solvent was n-hexane with the ratio of solid to liquid was 1 ∶ 15 (g/mL),treated by the ultrasonic power at 80 W for 2 h at 50 ℃.The extraction rate of Fructus Schisandrae Seed Oil was 29.94％.The content of different components was detected as 4.0％ of schizandrin A,1.95％ of schisandrin B and 0.13％ of schisandrin.The injury of the mice livers could be successfully induced by administration with 0.2％ concentrations of CCl4.The high,medium and low doses of Fructus Schisandrae Seed Oil could significantly reduce the MDA (P＜0.05 or P＜0.01) and increase the levels of SOD and GSH (P＜0.05).The HE staining results showed that schisandra seed oil could significantly inhibit the edema and necrosis of the mice livers.关键词
五味子/籽仁油/超声辅助提取/木脂素/保肝Key words
Fructus Schisandrae/seed oil/ultrasound-assisted extraction/lignans/hepatic protection引用本文复制引用
王小宁,郝晶,刘子晨,闫梦茹,张存劳..五味子油超声辅助提取工艺优化及其护肝作用[J].食品与机械,2018,34(1):135-139,172,6.基金项目
陕西省缺血性心血管疾病重点实验室开放基金(编号:2017ZDKF09) （编号:2017ZDKF09）
西安医学院大学生创新基金项目(编号:2016DXS1-19) （编号:2016DXS1-19）
