食品与机械2018，Vol.34Issue(1)：154-158,198,6.DOI:10.13652/j.issn.1003-5788.2018.01.031
祁白术生物活性物质及其抗氧化、抑菌能力研究
Study on bioactive components and antioxidant, antibacterial activities of Atractylodes macrocephala Koidz grown in Qimen
摘要
Abstract
The total contents of phenolics and flavonoids and the relativeantioxidant and antibacterial activities in different polarity fractions from Atractylodes macrocephala Koidz grown in Qimen (Qi Baizhu) were evaluated.The correlation between total phenolic,flavonoid contents and the antioxidant activities were analyzed.The main constituents of methanol crude extract were identified by gas chromatography-mass spectrometry (GC-MS).The results showed that there were significant differences in the contents of total phenolics and flavonoids,antioxidant and antibacterial activities among different polarity fractions including n-hexane,dichloromethane,ethyl acetate,n-butanol and water fractious (P ＜ 0.05).The ethyl acetate fraction had the highest contents of total phenolics [(139.45 ± 0.19) mg TA/g] and total flavonoids [(21.28± 2.28) mg QE/g].Moreover,it exhibited the strongest reducing power [(38.95-t-1.66) mg Vc/g],ABTS radical scavenging ability [(302.42± 7.51) mg Vc/g],and DPPH radical scavenging ability [(51.61±2.59) mg Vc/g].The contents of total phenolics and flavonoids demonstrated significant correlations with antioxidant activity (P＜0.05).The methanol extract,n-hexane and dichloromethane fractions exerted potent inhibitory effects on E.coli,S.aureus,B.subtilis and P.aeruginos.Hexane fraction presented the best antibacterial effect,and its minimum inhibitory concentration against four common bacterial strains was 1 mg/mL.The methanol crude extract was analyzed by GC-MS,and eighteen compounds were identified,including phenols,alcohols,alkenes and esters,and the major constituents were found to be 3,3'-diamino-4,4'-dihydroxybiphenyl (33.47％),β-eudesmol (28.33％),β-guaiene (5.91 ％),and 3,3,5,6,7-pentame-thyl-2 H-inden 1-one (4.70 ％).关键词
祁白术/生物活性物质/抗氧化/抑菌/相关性分析Key words
Qi Baizhu/bioactive components/antioxidant/antibacterial/correlation analysis引用本文复制引用
吴永祥,江海涛,崔朋,方建新,王卫东,胡长玉..祁白术生物活性物质及其抗氧化、抑菌能力研究[J].食品与机械,2018,34(1):154-158,198,6.基金项目
安徽省留学回国人员创新项目择优资助计划重点项目(编号:2017srst1) （编号:2017srst1）
校企合作项目(编号:hxkt20170015,hxkt20170016) （编号:hxkt20170015,hxkt20170016）
安徽省高校自然科学研究重点项目(编号:KJ2017A398) （编号:KJ2017A398）
