中国食物与营养2018，Vol.24Issue(4)：66-70,28,6.
核桃多肽对高糖诱导HepG2胰岛素抵抗模型的影响
Effect of Walnut Peptide on HepG2 Insulin Resistance Induced by High Glucose
李丽琪 1徐华容 2刘阳 1李潇 2蔡依 1阮金兰2
作者信息
- 1. 武昌理工学院生命科学院/生物多肽糖尿病药物湖北省协同创新中心,武汉430223
- 2. 武汉工程大学化工与制药学院,武汉430073
- 折叠
摘要
Abstract
[Objective] To investigate the hypoglycemic mechanism of walnut polypeptides at the cellular level and provide scientific basis for walnut resource development and utilization.[Method] HT polypeptide in the laboratory extraction,separation and purification of HT-1,based on HT-2,screened by GLUT4 transmembrane activity screening of L6myotubes glucose uptake activity of HepG2 cells induced by high glucose and insulin resistance in experimental diabetes model,biological inquiry of walnut polypeptide activity and hypoglycemic mechanism.[Result] HT,HT-1 and HT-2 polypeptides had pro GLUT4 transmembrane activity,the dose-response curve trend of distribution was better for HT-2,15min response,reached the peak at 30 min,in 25 min cell membrane GLUT4 increased 1 times;glucose uptake activity was better,the uptake rates were 1.16,1.06 and 1.36.The expression level of HT-2 and IR;beta,IRS-1 phosphorylation and GLUT2 protein increased in a concentration dependent manner,showed that HT-2 through the insulin signal transduction pathway to improve glucose metabolism.[Conclusion] The hypoglycemic activity of HT polypeptide,HT-1 and HT-2 is better,and the effect of HT-2 is obvious,and the glucose metabolism is improved by insulin signaling pathway.关键词
核桃多肽/胰岛素抵抗/降糖活性Key words
walnut polypeptide/insulin resistance/hypoglycemic activity引用本文复制引用
李丽琪,徐华容,刘阳,李潇,蔡依,阮金兰..核桃多肽对高糖诱导HepG2胰岛素抵抗模型的影响[J].中国食物与营养,2018,24(4):66-70,28,6.
