食品科学2018，Vol.39Issue(9)：7-12,6.DOI:10.7506/spkx1002-6630-201809002
酸性电解水对南美白对虾中多酚氧化酶活性的影响
Effect of Acidic Electrolyzed Water on Polyphenoloxidase Activity from Litopenaeus vannamei
摘要
Abstract
Recently, acidic electrolyzed water (AEW) has been applied in seafood preservation as a novel technology. It is reported that AEW has strong ability to delay the development of melanosis in Litopenaeus vannamei and extend its shelf-life. In this paper, polyphenoloxidase (PPO), an essential enzyme for melanosis in Litopenaeus vannamei , was extracted and purified. The effects and kinetics of AEW on purified PPO were then investigated. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, PPO from Litopenaeus vannamei was well purified. Kinetic studies revealed that AEW exhibited inhibitory effects on PPO with a dose-effect relationship. Moreover, AEW showed mixed-type inhibition on PPO with inhibitory constant Kivalue of 0.64 mmol/L. In conclusion, this study can provide a theoretical basis for further exploring the mechanism of AEW in food preservation.关键词
酸性电解水/南美白对虾/多酚氧化酶/混合型抑制/黑变Key words
acidic electrolyzed water/ Litopenaeus vannamei/ polyphenoloxidase/ mixed-type inhibition/ melanosis分类
轻工纺织引用本文复制引用
孙江萍,赵莉,俞文英,谢晶,潘迎捷,赵勇..酸性电解水对南美白对虾中多酚氧化酶活性的影响[J].食品科学,2018,39(9):7-12,6.基金项目
国家自然科学基金面上项目(31571917 （）
31671779) （）
上海市科委计划项目(14320502100) （14320502100）
上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号 （）
2015第4-8号 （）
2016第1-1号) （）
上海市教委曙光计划项目(15SG48) （15SG48）
