食品科学2018，Vol.39Issue(9)：127-133,7.DOI:10.7506/spkx1002-6630-201809020
草苁蓉多糖对氧化应激所致血管内皮细胞凋亡的抑制作用
Inhibition of Boschniakia rossica Polysaccharides on Oxidative Stress-Induced Apoptosis in Vascular Endothelial Cells
摘要
Abstract
Objective: To study the inhibitory effect of Boschniakia rossica polysaccharides (BRPS) on oxidative stress-induced apoptosis in vascular endothelial cells in vitro . Methods: A cellular model was established by treating human umbilical vein endothelial cells with tert-butyl hydroperoxide (tBHP). Cell viability was tested by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione (GSH) were measured spectrophotometrically. The reactive oxygen species (ROS) was observed using the fluorescent probe dichlorofluorescein diacetate (DCFH-DA), the mitochondrial membrane potential (ΔΨm) was evaluated by JC-1 staining, and the apoptotic changes were detected by Hoechst staining and TdT-mediated dUTP nick end labeling (TUNEL) assay. The protein expression of Bax, Bcl-2, cytochrome c (Cyt c), truncated Bid (tBid), caspase-3, caspase-8 and caspase-9 were determined by Western blotting. Results: Pretreatment with BRPS significantly increased cell viability, reduced ROS level, inhibited MDA formation, increased SOD activity and GSH level, elevated ΔΨm, and suppressed tBHP induced apoptosis in vascular endothelial cells. Western blotting results showed that BRPS reduced mitochondrial tBid, reduced cytoplasmic Cyt c and Bax/Bcl-2 ratio, and suppressed the activation of caspase-3, caspase-8 and caspase-9 in vascular endothelial cells. Conclusion: BRPS has an inhibitory effect on oxidative stress-induced apoptosis in vascular endothelial cells in vitro , likely through inhibiting mitochondrial apoptosis and death receptor pathways.关键词
草苁蓉/多糖/血管内皮细胞/叔丁基过氧化氢/凋亡Key words
Boschniakia rossica/ polysaccharides/ vascular endothelial cells/ tert-butyl hydroperoxide/ apoptosis分类
轻工纺织引用本文复制引用
崔香丹,何鑫,朱洁波,刘莉园,全吉淑,尹学哲..草苁蓉多糖对氧化应激所致血管内皮细胞凋亡的抑制作用[J].食品科学,2018,39(9):127-133,7.基金项目
国家自然科学基金地区科学基金项目(81760659 （）
81360651 （）
81160539) （）
