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苯并噻二唑处理对柑橘果实采后病害控制效果及机制分析

刘晓佳 薛耀碧 周雅涵 姚世响 邓丽莉 曾凯芳

食品科学2018,Vol.39Issue(9):215-223,9.
食品科学2018,Vol.39Issue(9):215-223,9.DOI:10.7506/spkx1002-6630-201809033

苯并噻二唑处理对柑橘果实采后病害控制效果及机制分析

Control and Mechanism of Postharvest Diseases in Citrus Fruits by Benzothiadiazole Treatment

刘晓佳 1薛耀碧 1周雅涵 1姚世响 1邓丽莉 1曾凯芳2

作者信息

  • 1. 西南大学食品科学学院,重庆 400715
  • 2. 重庆市特色食品工程技术研究中心,重庆 400715
  • 折叠

摘要

Abstract

(Fengqi Navel) orange fruits (Citrus sinensis L. Osbeck) were used as experimental subjects to evaluate the efficacy of different concentrations of benzothiadiazole (BTH) in controlling lesion diameters and disease incidence in citrus fruits inoculated with Penicillium italicum, Penicillium digitatum and Colletotrichum gloeosporides separately in the same and different wounds. At the same time, the optimum concentration of BTH to control these diseases was determined. The effects of BTH wound inoculation and dipping treatment on endogenous salicylic acid (SA) content and disease resistance-related enzymes activities in citrus peel were also investigated. The results indicated that BTH treatment at three concentrations (0.25, 0.50, 1.00 mmol/L) could reduce lesion diameters of citrus fruits inoculated with three pathogens and the best effect was observed at 0.50 mmol/L. All these three treatments controlled disease incidence at the early stage of disease, but the effect was marginal at the late stage. By comparison, BTH at 0.50 mmol/L was most effective. BTH wound inoculation at the optimum concentration could increase the content of endogenous SA in the peel. BTH dipping treatment had no significant effect on the content of endogenous free SA in the peel, but could promote the accumulation of endogenous bound SA content. Both BTH treatments could increase the activities of β-1,3-glucanase (GLU), chitinase (CHI), phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in the peel. Therefore, BTH could be effective in inducing postharvest disease resistance in citrus fruits.

关键词

苯并噻二唑/柑橘果实/侵染性病害/诱导抗病性

Key words

benzothiadiazole/ citrus fruit/ infectious diseases/ induced resistance

分类

轻工纺织

引用本文复制引用

刘晓佳,薛耀碧,周雅涵,姚世响,邓丽莉,曾凯芳..苯并噻二唑处理对柑橘果实采后病害控制效果及机制分析[J].食品科学,2018,39(9):215-223,9.

基金项目

"十二五"国家科技支撑计划项目(2015BAD16B07) (2015BAD16B07)

国家自然科学基金面上项目(31471631) (31471631)

重庆市社会事业与民生保障科技创新专项(cstc2016shms-ztzx80005) (cstc2016shms-ztzx80005)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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