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香菇β-葡聚糖的提取及其对淀粉消化性的影响

陈忠秋 冯涛 庄海宁 杨焱 张劲松

食品科学2018,Vol.39Issue(10):71-77,7.
食品科学2018,Vol.39Issue(10):71-77,7.DOI:10.7506/spkx1002-6630-201810012

香菇β-葡聚糖的提取及其对淀粉消化性的影响

Extraction of β-Glucan from Lentinus edodes and Its Effect on Starch Digestibility

陈忠秋 1冯涛 1庄海宁 2杨焱 2张劲松2

作者信息

  • 1. 上海应用技术大学香料香精技术与工程学院,上海 201418
  • 2. 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海 201403
  • 折叠

摘要

Abstract

Lentinus edodes β-glucan (LEBG) was extracted from the fruiting bodies of Lentinus edodes. The average molecular weight of LEBG was measured to be 1.868 × 106Da. Monosaccharide composition analysis showed that LEBG was composed of arabinose, xylose, mannose, glucose and galactose with a molar ratio of 0.72:1.61:2.61:92.75:2.34. Infrared (IR) spectroscopy showed that LEBG was a polysaccharide containing pyranose ring. Addition of LEBG to wheat starch and corn starch increased both their loss modulus and storage modulus. Dynamic rheological measurements revealed the interaction between LEBG and starch. During in vitro digestion, the rates of enzymatic hydrolysis of wheat starch and corn starch supplemented with LEBG were significantly lower than those without LEBG. This difference may be due to the fact that LEBG and starch are intertwined, enhancing the structure of the gel network. At the same time, both starches with LEBG showed lower levels of rapidly digestible starch and predicted glycemic index and higher levels of slowly digestible starch and resistant starch, indicating that LEBG has an inhibitory effect on the in vitro digestion of starch. In the visualization experiment, we further confirmed that starch was wrapped by LEBG. Ultrafine Lentinus edodes powder could also inhibit in vitro starch digestion due to its β-glucan. This study has indicated that LEBG shows promise for developing low-glycemic-index starchy foods.

关键词

香菇β-葡聚糖/流变/淀粉/体外消化/血糖指数预测

Key words

Lentinus edodes β-glucan/ rheological properties/ starch/ in vitro digestibility/ predicted glycemic index

分类

轻工纺织

引用本文复制引用

陈忠秋,冯涛,庄海宁,杨焱,张劲松..香菇β-葡聚糖的提取及其对淀粉消化性的影响[J].食品科学,2018,39(10):71-77,7.

基金项目

2015年度上海市市级农口系统青年人才成长计划项目(沪农青字(2015)第1-8号) (沪农青字(2015)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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