食品科学2018,Vol.39Issue(10):78-83,6.DOI:10.7506/spkx1002-6630-201810013
香榧不同部位提取物的抗氧化和酶抑制活性比较分析
Comparison of Antioxidant Activity and Enzyme Inhibitory Activity of Different Plant Parts of Torreya grandis cv. Merrillii
摘要
Abstract
This study aimed to compare the phytochemical constituents, antioxidant activity, anti-diabetes activity and anti-gout potential of the leaf, stem, aril, seed shell and seed kernel of Torreya grandis cv. Merrillii by analyzing the contents of phenolics, flavonoids and condensed tannins, as well as radical scavenging ability and inhibitory activities against α-glucosidase and xanthine oxidase. The results indicated that the main bioactive constituents, antioxidant activity and α-glucosidase inhibitory activity of different plant parts varied significantly (P<0.05). The kernel and leaf extracts had the highest total phenolic content (25.28 mg GAE/g) and condensed tannins content (1.10 mg CE/g), respectively. The highest content of flavonoids was found in the stem and aril extracts (P > 0.05). Bioactivity analysis revealed that the kernel extract showed the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS+·) scavenging ability, inhibitory activities against α-glucosidase and xanthine oxidase, and its α-glucosidase inhibitory activity (IC50= 0.14 mg/mL) was much higher than that of the positive control acarbose (IC50=1.29 mg/mL). Thus, it can be concluded that the seed kernel of Torreya grandis cv. Merrillii is a better resource of natural antioxidants and inhibitors of both α-glucosidase and xanthine oxidase than its leaf, stem, aril and seed shell.关键词
香榧/活性成分/抗氧化/α-葡萄糖苷酶/黄嘌呤氧化酶Key words
Torreya grandis cv. Merrillii/ bioactive constituents/ antioxidant activity/ α-glucosidase/ xanthine oxidase分类
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张露,刘鹏飞,涂宗财,谢星,张恕雅,沙小梅,王辉,戴花剑..香榧不同部位提取物的抗氧化和酶抑制活性比较分析[J].食品科学,2018,39(10):78-83,6.基金项目
江西省青年科学基金项目(20171BAB214037) (20171BAB214037)
江西省教育厅项目(GJJ150331) (GJJ150331)