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剁辣椒中优良乳酸菌的分离鉴定及其生物学特性分析

叶陵 李勇 王蓉蓉 刘成国 蒋立文 邓放明 周辉

食品科学2018,Vol.39Issue(10):112-117,6.
食品科学2018,Vol.39Issue(10):112-117,6.DOI:10.7506/spkx1002-6630-201810018

剁辣椒中优良乳酸菌的分离鉴定及其生物学特性分析

Isolation, Identification and Biological Characteristics of Excellent Lactic Acid Bacteria from Fermented Hot Pepper

叶陵 1李勇 2王蓉蓉 1刘成国 1蒋立文 1邓放明 1周辉1

作者信息

  • 1. 湖南农业大学食品科学技术学院,湖南省食品科学与生物技术重点实验室,湖南 长沙 410128
  • 2. 张家界灵洁绿色食品有限公司,湖南 张家界 427000
  • 折叠

摘要

Abstract

Lactic acid bacteria were isolated from fermented pepper and five strains of Lactobacillus sp. were screened out based on their acid production and nitrite depletion ability. By biochemical tests and genetic analysis based on 16S rDNA sequence, strains W-4, Y-5 and Y-12 were identified as L. plantarum, and the other two strains Y-25 and Y-31 were identified as L. brevis. The stress response indicated that the L. plantarum survived better under acidic and osmotic stresses as compared with L. brevis, and L. plantarum W-4 had the strongest tolerance ability. All five strains were sensitive to erythromycin, ampicillin, gentamicin, tetracycline and penicillin G.

关键词

剁辣椒/乳杆菌/鉴定/特性

Key words

fermented hot pepper/ Lactobacillus/ identification/ characteristics

分类

轻工纺织

引用本文复制引用

叶陵,李勇,王蓉蓉,刘成国,蒋立文,邓放明,周辉..剁辣椒中优良乳酸菌的分离鉴定及其生物学特性分析[J].食品科学,2018,39(10):112-117,6.

基金项目

国家自然科学基金面上项目(31571811) (31571811)

国家自然科学基金青年科学基金项目(31601525) (31601525)

湖南省教育厅科学研究项目(16K043) (16K043)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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