食品科学2018，Vol.39Issue(10)：326-332,7.DOI:10.7506/spkx1002-6630-201810049
基于微型DNA条形码的多种动物源性成分的鉴定
Mini-DNA Barcode for Identification of Multiple Animal-Derived Ingredients
摘要
Abstract
Based on the standard DNA barcodes of 51 animal species, a new pair of universal primers for mini-DNA barcode was designed and combined with clone sequencing to develop an accurate and efficient method for identifying multiple animal derived ingredients. Eleven common edible meat species were identified by the mini-DNA barcode to verify its accuracy in comparison with the results of standard DNA barcode. A mixture in equal proportion of the 11 species was used to evaluate the colon sequencing method. The results showed that the designed primers had favorable universality among 11 species with different levels of sequence homology with primers. Thus, 11 meat species could be accurately identified with the mini-barcode, which showed high consistency to the standard DNA barcode. By clone sequencing, nine species from mixed samples were detected. Furthermore, the reasons why not all the species were detected were discussed. The results of this study can lay a foundation for combining mini-DNA barcode and next generation sequencing technique for efficient species identification in mixed meat.关键词
微型DNA条形码/物种鉴定/DNA条形码技术Key words
mini-DNA barcode/ species identification/ DNA barcode technique分类
生物科学引用本文复制引用
潘艳仪,邱德义,陈健,杨维东,岳巧云..基于微型DNA条形码的多种动物源性成分的鉴定[J].食品科学,2018,39(10):326-332,7.基金项目
"十三五"国家重点研发计划重点专项(2016YFF0203205) （2016YFF0203205）
国家质量监督检验检疫总局科技计划项目(2017IK165) （2017IK165）
广东检验检疫局科技计划项目(2017GDK63) （2017GDK63）
