食品科学2018，Vol.39Issue(11)：1-7,7.DOI:10.7506/spkx1002-6630-201811001
南极磷虾抗冻蛋白热滞活性的差示扫描量热法评价
Determination of Thermal Hysteresis Activity of Antifreeze Protein from Antarctic Krill (Euphausia superba ) by Differential Scanning Calorimetry
摘要
Abstract
In this study, we investigated whether differential scanning calorimetry (DSC) can be used to detect thermal hysteresis activity (THA) of antifreeze proteins (AFPs) from Antarctic krill. Important experimental conditions, such as cooling rate, sample concentration, ice crystal content and buffer system, were optimized. The results showed that the molecular weight of AFPs was about 76 kDa and its THA was 1.76 ℃. A cooling rate of 1.00 ℃/min, a sample concentration of 1.0 mg/mL and an ice crystal content ranging from 10% to 15% were found to be the optimal conditions. THA was greatly affected by different buffer systems. This study led us to conclude that DSC can be effectively used for the evaluation of THA of AFPs.关键词
南极磷虾/抗冻蛋白/热滞活性/差示扫描量热法/评价Key words
Euphausia superba/ antifreeze protein/ thermal hysteresis activity/ differential scanning calorimetry/ evaluation分类
轻工纺织引用本文复制引用
马庆保,刘志东..南极磷虾抗冻蛋白热滞活性的差示扫描量热法评价[J].食品科学,2018,39(11):1-7,7.基金项目
国家自然科学基金面上项目(31471687) （31471687）
上海市自然科学基金项目(13ZR1449900) （13ZR1449900）
上海市科技兴农项目(沪农科攻字(2015)第5-5号) （沪农科攻字(2015）
中央级公益性科研院所基本科研业务费专项资金项目(2016HY-ZD0903 （）
2016HY-ZD1003) （）
