食品科学2018，Vol.39Issue(11)：119-125,7.DOI:10.7506/spkx1002-6630-201811019
提取方法对产朊假丝酵母β-葡聚糖性质的影响
Physicochemical and Moisturizing Properties of β-Glucan from Candida utilis Extracted by Different Methods
摘要
Abstract
Three different methods: high pressure microfluidization, ultrasonic, dual enzymatic hydrolysis were employed to extract β-glucan from the cell walls of the yeast Candida utilis and the obtained extracts were designated as H-YG, U-YG and E-YG, respectively. The yields, purities, molecular mass distributions, and monosaccharide compositions of these β-glucans were compared. We also evaluated the effect of three extraction methods on the antioxidant activity and moisturizing properties of β-glucans. The results showed that the purities of β-glucan extracted by three methods were all above 96% and high pressure microfluidization gave the highest yield and purity of β-glucan of 19.6% and 97.5%, respectively. UV spectra showed that none of these β-glucans contained nucleic acids, proteins or other impurities. Monosaccharide composition analysis showed that all three β-glucans were homogenous polysaccharides composed of only glucose. The molecular mass distribution and the average molecular radius were measured by high-performance size exclusion chromatography and multi-angle laser light scattering, and the results indicated that the weight average molecular masses of H-YG, U-YG and E-YG were 1.611 ×106, 4.763 × 106and 2.661 × 105g/mol, the polydispersity coefficients were 1.025, 1.686 and 1.362 and the average molecular radii were 67.5, 33.3 and 43.7 nm, respectively. All three β-glucans showed DPPH radical scavenging capacity, suggesting their antioxidant potential. Furthermore, they had significant moisturizing properties in vitro, and the moisturizing effect of H-YG was better than that of glycerol in 8-24 h. All these β-glucans especially H-YG significantly contributed to promoting the generation of endogenous natural moisturizing factor. These findings demonstrated that different extraction methods affected physicochemical properties and bioactivity of yeast β-glucan, and could be applied to various fields according to their characteristics.关键词
产朊假丝酵母/酵母β-葡聚糖/提取方法/抗氧化活性/保湿性能Key words
Candida utilis/ yeast β-glucan/ extraction method/ antioxidant activity/ moisturizing performance分类
生物科学引用本文复制引用
马 霞,刘蓝天,沈 丽,邵 丽..提取方法对产朊假丝酵母β-葡聚糖性质的影响[J].食品科学,2018,39(11):119-125,7.基金项目
上海市联盟计划项目(LM201753) （LM201753）
上海高校青年教师培养资助计划项目(ZZZZyyx16015) （ZZZZyyx16015）
