食品科学2018，Vol.39Issue(11)：159-164,6.DOI:10.7506/spkx1002-6630-201811025
北极海洋红球菌B7740(Rhodococcus sp.)产类胡萝卜素和类异戊二烯醌的抗氧化、抗增殖活性
Antioxidant and Antiproliferative Activity of Carotenoids and Isoprenoid Quinones from Rhodococcus sp. B7740
摘要
Abstract
In this paper, the antioxidant and antiproliferative activity of a carotenoid and isoprenoid quinone-rich extract (B7CIQE) from Rhodococcus sp. B7740 were determined. The in vitro antioxidant activity was evaluated by β-carotene bleaching, lipid peroxidation inhibition, protein oxidation inhibition and oxidative DNA cleavage inhibition assays, and the antiproliferative activity against HepG2 human liver cancer and KB human oral cancer cells and the cellular antioxidant activity were investigated as well. Three common carotenoids from higher plants, β-carotene, lutein and lycoypene, were used as controls. The inhibition rate of β-carotene oxidation by different antioxidants was in the decreasing order of B7CIQE (70.20%) ＞ butylated hydroxytoluene (66.70%) ＞ epigallocatechin gallate (17.80%) ＞ lycopene (1.90%). Peanut oil with the addition of B7CIQE had the lowest initial oxidation temperature (175 ℃) followed by β-carotene (165 ℃), lutein (162 ℃) and lycopene (160 ℃). The inhibition rate of protein oxidation was in the decreasing order of B7CIQE (25.75%) ＞ β-carotene (24.97%) ＞ lutein (17.94%) ＞ lycopene (10.40%). The median effective concentration (EC50) against HepG2 cells was in the decreasing order of lutein (20.86 μg/mL) ＜ β-carotene (124.88 μg/mL) ＜ B7CIQE (126.34 μg/mL) ＜ lycopene (139.24 μg/mL), whereas the EC50against KB cells decreased in the following order: B7CIQE (25.14 μg/mL) ＜ lutein (64.29 μg/mL) ＜ lycopene (69.87 μg/mL) ＜ β-carotene (149.16 μg/mL). These results demonstrated that B7CIQE had superior antioxidant activity and antiproliferative effect when compared with plant-derived carotenoids.关键词
微生物产类胡萝卜素/微生物产类异戊二烯醌/红球菌B7740/抗氧化活性/抗增殖活性Key words
microbial carotenoids/ microbial isoprenoid quinones/ Rhodococcus sp. B7740/ antioxidant activity/ antiproliferative activity分类
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穆 青,陈亚淑,谢笔钧,杨季芳,陈吉刚,孙智达..北极海洋红球菌B7740(Rhodococcus sp.)产类胡萝卜素和类异戊二烯醌的抗氧化、抗增殖活性[J].食品科学,2018,39(11):159-164,6.基金项目
国家海洋公益性科研专项(201405015-5) （201405015-5）
宁波市科技局择优委托项目(2012C10038) （2012C10038）
