食品科学2018，Vol.39Issue(11)：196-200,5.DOI:10.7506/spkx1002-6630-201811031
降香内生真菌代谢产物对H2O2诱导的HepG2细胞氧化损伤保护作用
Protective Effect of an Endophytic Fungus from Dalbergia odorifera T. Chen against H2O2-Induced Oxidative Damage in HepG2 Cells
摘要
Abstract
In this study, a strain of endophytic fungus JXP21 with good antioxidant activity was isolated from the barks of Dalbergia odorifera T. Chen, and was identified as Alternaria alternata through ITS-rDNA sequence alignment and phylogenetic analysis, whose ITS-rDNA sequence was 100% similar with GenBank accession number KP979753.1. The compound genistein was detected as one of its metabolites by high performance liquid chromatography tandem mass spectrometry. The protective effect of the metabolites of JXP21 at different concentrations (0, 10, 20, 40 and 80 μg/mL) on oxidative damage induced by 200 μmol/L H2O2in HepG2 cells was investigated by measuring malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. The results showed that the fungal metabolites decreased the increase in MDA content from 4.65 to 3.68 nmol/mg protein, increased SOD activity from 6.88 to 8.64 U/mg pro and GSH-Px activity from 8.45 to 9.68 U/mg pro. In conclusion, the results of this study indicated that the endophytic fungal metabolites can protect against oxidative damage induced by H2O2.关键词
降香/内生真菌/超氧化物歧化酶/谷胱甘肽过氧化物酶/丙二醛Key words
Dalbergia odorifera T. Chen/ endophytic fungi/ superoxide dismutase/ glutathione peroxidase/ malondialdehyde分类
生物科学引用本文复制引用
高原,王福玲,贾琦,刘微,周艳岩,张晓萌,咸玥桐..降香内生真菌代谢产物对H2O2诱导的HepG2细胞氧化损伤保护作用[J].食品科学,2018,39(11):196-200,5.基金项目
黑龙江省教育厅科学技术研究项目(12541204) （12541204）
