食品科学2018，Vol.39Issue(12)：26-32,7.DOI:10.7506/spkx1002-6630-201812005
鲍鱼肌肉多糖的性质及抗肿瘤活性
Physicochemical Properties and Antitumor Activity of Polysaccharides from Abalone Muscle
摘要
Abstract
Abalone muscle polysaccharide (AMP) was prepared from the muscle of Haliotis discus hannai Ino by protease hydrolysis combined with fractional precipitation with gradient concentrations of ethanol. Three fractions (AMP-1, AMP-2 and AMP-3) were obtained by DEAE-52 column chromatography. Their physicochemical properties and antitumor activity were investigated. The results of chemical composition suggested that the total sugar content of AMP was 83.9%, while the total sugar content of AMP-1 was 83.8%, which was higher than that of AMP-2 (62.5%) and AMP-3 (31.6%). Monosaccharide composition analysis suggested that AMP was mainly composed of Glc, AMP-1 was almost exclusively composed of Glc, and AMP-2 and AMP-3 were mainly composed of Glc and Gal together with hexosamine. Based on the results of chemical composition as well as ultraviolet spectroscopic and Fourier transform infrared spectroscopic analyses, AMP-1 was α-pyranose with high purity, while AMP-2 and AMP-3 might be glycoprotein. The results of iodine staining indicated that AMP-1 was a non-starch glucan. Scanning electron microscopy showed that AMP and AMP-1 had a lamellar structure, while AMP-2 and AMP-3 contained many spherical particles. Furthermore, AMP and AMP-1 showed a strong inhibitory activity against both human breast cancer cell line MDA-MB-231 and human liver cancer cell line HepG2.关键词
鲍鱼肌肉/多糖/化学组成/单糖组成/表面形态/抗肿瘤活性Key words
abalone muscle/polysaccharide/chemical composition/monosaccharide composition/surface morphology/antitumor activity分类
化学化工引用本文复制引用
王军玲,魏配晓,邱绪建,翁武银..鲍鱼肌肉多糖的性质及抗肿瘤活性[J].食品科学,2018,39(12):26-32,7.基金项目
国家自然科学基金面上项目(31571835) （31571835）
国家海洋公益性行业科研专项(201405016) （201405016）
