食品科学2018，Vol.39Issue(12)：161-166,6.DOI:10.7506/spkx1002-6630-201812025
动物双歧杆菌细菌素bifidocin A群体感应合成调控行为分析
Quorum-Sensing Regulation Behavior of Bifidocin A Production in Bifidobacterium animalis
摘要
Abstract
Bifidocin A is a novel bacteriocin produced by Bifidobacterium animalis BB04 with antimicrobial activity against a wide range of foodborne bacteria. The objective of this study was to investigate the quorum-sensing regulation behavior of bifidocin A production in B. animalis BB04. The correlation between cell density and bacteriocin production was analyzed by monitoring the cell growth and bacteriocin antimicrobial activity during the fermentation process. Using a low bacteriocin culture model system, the presence of auto-inducing peptide and quorum-sensing system related to bacteriocin production were analyzed. The auto-inducing peptide was purified by ultrafiltration, Sephadex G25 chromatography and HPLC, and its molecular mass was determined by matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS). Results showed that bifidocin A began to be synthesized when the cell density reached 7.31 (lg (CFU/mL)), and the bacteriocin production depended on cell density. In the low bacteriocin culture model system, the culture temperature was 37 ℃, the initial pH value of culture medium was 5, the concentration of culture medium was 1/10 modified MRS, the inoculum quantity was 1%, and the incubation time was 24 h. The auto-inducing peptide was detected in the fermentation supernatant and it mediated the regulation of bacteriocin production by the quorum-sensing system. The molecular mass of the purified auto-inducing peptide was 3 587.253 Da.关键词
细菌素/双歧杆菌/群体感应/自诱导肽/合成/调控Key words
bacteriocin/Bifidobacterium spp./quorum-sensing/auto-inducing peptide/production/regulation分类
轻工纺织引用本文复制引用
刘国荣,任桂美,李雪,王成涛..动物双歧杆菌细菌素bifidocin A群体感应合成调控行为分析[J].食品科学,2018,39(12):161-166,6.基金项目
国家自然科学基金面上项目(31671832) （31671832）
北京市属高校高水平教师队伍建设支持计划青年拔尖人才培育计划项目(CIT&TCD201704034) （CIT&TCD201704034）
北京市科技计划项目(Z171100002217019) （Z171100002217019）
"十三五"国家重点研发计划重点专项(2016YFD0400502-02) （2016YFD0400502-02）
