食品科学2018，Vol.39Issue(12)：191-198,8.DOI:10.7506/spkx1002-6630-201812030
HPGFC双柱串联检测右旋糖酐蔗糖酶催化产物及右旋糖酐聚合过程
Determination of Dextransucrase-Catalyzed Reaction Products of Sucrose by High Performance Gel Filtration Chromatography with Tandem Two Columns and Analysis of the Dextran Polymerization Process
摘要
Abstract
A precise method for the determination of changes in the molecular mass of dextran and the quantification of sucrose, fructose and glucose concentration during the dextransucrase-catalyzed reaction of sucrose was presented in this paper. High performance gel filtration chromatography (HPGFC) with one column and with two tandem columns were compared for their efficiencies in the determination of dextran standard and the catalytic reaction products in order to develop a robust method to measure the molecular mass of dextran. By dextransucrase combined with two-stage membrane treatment, we investigated the mechanism underlying dextran synthesis. It was found that the synthesis process was continuous aggregation of glucosyl group at the active site of dextransucrase until reaching a specific molecular mass (> 106Da) before detachment rather than stepwise aggregation and then detachment.关键词
高效凝胶过滤色谱/双柱串联/右旋糖酐分子质量/聚合过程Key words
high performance gel filtration chromatography (HPGFC)/two tandem columns/molecular mass of dextran/aggregation process分类
轻工纺织引用本文复制引用
黄双霞,侯殿志,陈华磊,于玥,陈山..HPGFC双柱串联检测右旋糖酐蔗糖酶催化产物及右旋糖酐聚合过程[J].食品科学,2018,39(12):191-198,8.基金项目
国家自然科学基金地区科学基金项目(21768001) （21768001）
