食品科学2018，Vol.39Issue(12)：239-246,8.DOI:10.7506/spkx1002-6630-201812037
黑果腺肋花楸花色苷提取工艺优化及其抗氧化活性和组成鉴定
Optimization of Extraction of Anthocyanins from Berries of Aronia melanocarpa and Their Antioxidant Activity and Composition
摘要
Abstract
This study aimed to optimize the ultrasonic-assisted extraction of anthocyanins from Aronia melanocarpa and to determine the antioxidant capacity and constituents of the obtained extract. The optimization was carried out by response surface methodology. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS+·) scavenging capacity and Fe3+reducing ability of plasma (FRAP) assays were used to evaluate the antioxidant capacity. The anthocyanin components were analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the optimum extraction conditions were as follows: extraction temperature 60 ℃, ultrasonic power 100 W, solid-to-liquid 1:30 (g/mL), ultrasonic time 31 min, ethanol concentration 64%, and pH 2.0. Under these conditions, the content of anthocyanins was determined to be (3.61 ± 0.01) mg/g. The in vitro antioxidant activity of the anthocyanin extract was significantly higher than that of vitamin C at the same concentration. Six anthocyanins including two new compounds, dimer of cyanidin-3-hexoside and cyanidin-3,5-dihexoside, were detected in the extract.关键词
黑果腺肋花楸/花色苷/响应面/抗氧化/高效液相色谱-质谱联用Key words
Aronia melanocarpa /anthocyanins/response surface methodology/antioxidant activity/high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)分类
轻工纺织引用本文复制引用
位路路,林杨,王月华,孟宪军..黑果腺肋花楸花色苷提取工艺优化及其抗氧化活性和组成鉴定[J].食品科学,2018,39(12):239-246,8.基金项目
"十三五"国家重点研发计划重点专项(2017YFD0400704-4) （2017YFD0400704-4）
