食品科学2018，Vol.39Issue(12)：254-261,8.DOI:10.7506/spkx1002-6630-201812039
核桃多肽-苦荞-藜麦复合粉制备工艺及体外消化和抗氧化功能特性分析
Preparation, Digestibility and Antioxidant Activity in Vitro of Composite Powder of Walnut Peptide-Buckwheat-Quinoa
摘要
Abstract
In this paper, composite powders of walnut peptide, buckwheat and quinoa at different ratios were prepared. The optimal ratio was determined by evaluating their protein nutritional value based on the calculated score of ratio coefficient of amino acid (SRCAA). The conditions for liquefaction and saccharification of buckwheat and quinoa before being blended with walnut peptide were optimized by one-factor-at-a-time and orthogonal array design methods. The composite powder of walnut peptide, buckwheat and quinoa was obtained by spray drying and its digestbility and antioxidant activity in vitro were studied. The results showed that the optimal ratio of walnut peptide to buckwheat to quinoa was 0.7:13:5 (m/m), and the SRCAA of this combination was 95.15%, which was closed to the reference protein pattern. The optimal liquefaction conditions were α-amylase 7 U/g, 60 ℃, and digestion time 50 min, and the optimal saccharification conditions were β-amylase 150 U/g, 55 ℃, and digestion time 2.5 h. Under these conditions, the concentration of reducing sugar was 20.03 mg/mL. The contents of peptide, reducing sugar, flavonoid and polyphenol were increased during simulated in vitro digestion; especially during simulated intestinal digestion, the content of rapidly digestible starch (RDS) was 64.19% and the content of slowly digestible starch (SDS) was 27.12%, while the content of resistant starch (RS) was only 8.70%, which indicated that starch was more thoroughly hydrolyzed after gastrointestinal digestion. The antioxidant activities in vitro of the composite powder was proved by its reducing power (IC50= 5.04 mg/mL), scavenging activity against DPPH radical (IC50= 0.67 mg/mL), hydroxyl radical (IC50= 62.32 mg/mL) and superoxide anion radical (IC50= 13.07 mg/mL) and total antioxidant capacity (27.63 U/mL). To conclude, the composite powder of walnut peptide, buckwheat and quinoa was not only easy to digest and absorb but also had antioxidant activity.关键词
氨基酸评价/液化/糖化/体外模拟消化/抗氧化Key words
amino acid evaluation/liquefaction/saccharification/in vitro digestion/antioxidant activity分类
轻工纺织引用本文复制引用
陈树俊,李乐,胡洁,徐晓霞,石玥,李佳益,张君梅,王翠连..核桃多肽-苦荞-藜麦复合粉制备工艺及体外消化和抗氧化功能特性分析[J].食品科学,2018,39(12):254-261,8.基金项目
山西省重点研发计划项目(201603D221033-1) （201603D221033-1）
