食品科学2018，Vol.39Issue(12)：308-312,5.DOI:10.7506/spkx1002-6630-201812047
高效液相色谱-串联质谱法检测鲜冻肉中违禁注水药物
Determination of Illegal Water-Retaining Drugs in Frozen Meat by UPLC-MS/MS
摘要
Abstract
An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of six illegal water-retaining drugs, atropine, lidocaine, procaine, anisodamine, scopolamine and salbutamol, in frozen meat. The samples were extracted with phosphate buffer, and the extract was cleaned up on an HLB cartridge. The analytes were detected in the multiple reaction monitoring mode and quantified by the external standard method. The results indicated that good linearity over the concentration range of 0.25–50 ng/mL was observed for all analytes with correlation coefficients greater than 0.99. The limits of detection (LOD) and quantification (LOQ) were 0.5 and 2.0 μg/kg, respectively. The average recoveries of the six drugs ranged from 78.07% to 96.76% with relative standard deviations (RSDs) of 0.4%–2.1% (n = 6). The method is sensitive, simple, rapid and accurate and can meet the requirements of practical analysis.关键词
注水药物/保水剂/高效液相色谱-串联质谱法Key words
illegal drugs/water-retaining agent/ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)分类
轻工纺织引用本文复制引用
李志刚,任南,马燕红,李莹莹,郭文萍..高效液相色谱-串联质谱法检测鲜冻肉中违禁注水药物[J].食品科学,2018,39(12):308-312,5.基金项目
北京市优秀人才培养资助项目(2016754154700G147) （2016754154700G147）
