现代食品科技2018，Vol.34Issue(4)：18-22,29,6.DOI:10.13982/j.mfst.1673-9078.2018.04.004
柑橘PGIP的B细胞抗原表位分析和原核表达
B Cell Epitopes Analysis and Prokaryotic Expression of PGIP in Citrus
摘要
Abstract
Citrus is an important horticulture. However consumption of citrus fruits could cause allergen for some people. With the method of Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz, the antigenicity, hydrophilicity, protein surface accessibility and flexibility of citrus allergen protein PGIP (polygalacturonase inhibitor protein) were predicted. Meanwhile PET-28 a-PGIP vector was constructed, and then transfer to Escherichia coli. The target gene was induced and expressed. The results showed: When the secondary structure of amino acid was β-angle and irregular curl, the antigen index＞0, the flexibility＞0, the hydrophilicity＞0, the protein surface accessibility＞1 amino acid region, the sequences of the B cell epitope binding amino acid were 1922, 3741, 4952, 6869, 117120, 163164, 173177, 221226, 236240. By estimating the amino acid region, it provides support for finding the best possible region of the B cell epitope of citrus allergen protein PGIP, and also provides a theoretical basis for the further study of citrus allergen protein PGIP.关键词
柑橘/多聚半乳糖醛酸酶抑制蛋白/B细胞表位Key words
citrus/polygalacturonase inhibitory protein/cell epitope引用本文复制引用
陈波,罗庆华,谭雅芹,闫慧清..柑橘PGIP的B细胞抗原表位分析和原核表达[J].现代食品科技,2018,34(4):18-22,29,6.基金项目
国家自然科学基金资助项目(31660554) （31660554）
博士科研启动项目(11904-0514156、11904-0514157) （11904-0514156、11904-0514157）
"通识教育基础课程-营养与健康"课程团队项目和品牌团队(KT2016015B) （KT2016015B）
