现代食品科技2018，Vol.34Issue(4)：94-101,87,9.DOI:10.13982/j.mfst.1673-9078.2018.04.016
桉叶提取物中抗氧化活性物分离纯化、结构鉴定及其活性的研究
Identification, Isolation and Purification of the bioactive Compounds from Eucalyptus Leave Extract and theirs Antioxidant Activity
摘要
Abstract
To further elucidate antioxidant compounds of Eucalyptus leave extract, four compounds were isolated and purified from the fraction P95 of eucalyptus leave extract by medium-pressure silica gel column chromatography and preparative high performance liquid chromatography (HPLC). And three of the compounds were identified as hyperoside, guava glycosides and quercetin-3-oalpha-pyranol-2 "-gallate, by comparison of their 1 H-NMR and 13 C-NMR data. Then 1, 1-diphenyl-2-picrylhydrazyl (DPPH·), 2, 2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+·), and oxygen radical absorbance capacity (ORAC) assays were used for analyzing the antioxidant activity of the compounds. Among the DPPH and ORAC methods, guava glycosides showed the highest antioxidant activity, and quercetin-3-o-alpha-pyranol-2"-gallate showed the lowest antioxidant capacity. Hyperoside had the highest antioxidant activity, and guava glycosides had the lowest in ABTS+·assay. All of three compounds showed higher antioxidant activity than trolox, and they were natural antioxidants with better development prospects. This study provided a theoretical guidance for the comprehensive development of the Eucalyptus leaves.关键词
桉叶/DPPH自由基清除能力/氧自由基吸收Key words
eucalyptus leaves/DPPH free radical scavenging ability/oxygen free radical absorption引用本文复制引用
陈洪璋,李伟,肖苏尧,曹庸,陈运娇..桉叶提取物中抗氧化活性物分离纯化、结构鉴定及其活性的研究[J].现代食品科技,2018,34(4):94-101,87,9.基金项目
国家重点研发计划-人工林非木质林产资源高质化利用技术创新(2016YFD0600806) （2016YFD0600806）
广东省自然科学基金博士启动项目(2015A030310118) （2015A030310118）
广东省科技计划项目(2017A020208042) （2017A020208042）
