现代食品科技2018，Vol.34Issue(4)：150-158,241,10.DOI:10.13982/j.mfst.1673-9078.2018.04.024
三角褐指藻跑道池规模化培养及岩藻黄素积累条件的优化
Scaling-up Cultivation of Phaeodactylum tricornutum in Open Raceway Pond and Optimization of the Culture Conditions for Fucoxanthin Accumulation
摘要
Abstract
As a kind of carotenoid which existed in various marine microalgae, fucoxanthin had many biological activities including scavenging free radicals, anti-cancer, anti-obesity and other physiological activities, especially in anti-tumor and slimming effect. Phaeodactylum triconutum (P. tricornutum) had the capability to accumulate plenty of fucoxanthin, while the environment and nutritional factors have a significant effect on the growth of microalgae and the content of fucoxanthin. In this study, effects of different salinity (20, 25, 30), pH values (7. 5, 8. 0, 8. 5) and nitrogen source types (urea, ammonium bicarbonate) on the OD value, cell concentration, dry cell weight, fucoxanthin content of P. tricornutum were investigated in outdoor mass culture. The results showed that maximum cell concentration was 6. 01×106/m L, which was obtained in the culture medium with salinity of 20. There was no significant effect of different salinity on the increase of fucoxanthin content of P. tricornutum. Optimal p H value of the culture medium for P. tricornutum was 8. 0. Maximum cell concentration and fucoxanthin content were 5. 8×106/m L and 17. 79 mg/g, respectively. Compared with ammonium bicarbonate, urea as a nitrogen source was more suitable for the growth of P. tricornutum and the final cell concentration reached 4. 1×106/m L. However, in terms of fucoxanthin accumulation, ammonium bicarbonate as nitrogen source was more advantageous since the maximum fucoxanthin content reached 9. 96 mg/g. Therefore, the optimum culture conditions in open raceway pond for P. tricornutum were summed up to be salinity of 20, pH 8. 0 and ammonium bicarbonate as the nitrogen source.关键词
三角褐指藻/环境因子/氮源/岩藻黄素Key words
Phaeodactylum tricornutum/environmental factor/nitrogen source/fucoxanthin引用本文复制引用
宋培钦,刘鹭,魏东..三角褐指藻跑道池规模化培养及岩藻黄素积累条件的优化[J].现代食品科技,2018,34(4):150-158,241,10.基金项目
广东省公益研究与能力建设项目(2015A020216003、2016A010105001) （2015A020216003、2016A010105001）
广东省海洋渔业科技与产业发展专项(A201401C01) （A201401C01）
广州市科技计划项目产学研协同创新重大专项(201704030084) （201704030084）
国家自然科学基金项目(31370383)资助 （31370383）
