现代食品科技2018，Vol.34Issue(4)：179-187,9.DOI:10.13982/j.mfst.1673-9078.2018.04.028
响应面分析法优化离子液体双水相提取香樟叶中总黄酮的工艺条件
Response Surface Methodology for Optimization of Extraction Process of Total Flavonoids from Camphor Leaves by an Ionic Liquid/Aqueous Two-phase System
摘要
Abstract
Flavonoids of camphor leaves was extracted by an ionic liquid/aqueous two-phase system in this paper. First, the stability of aqueous two-phase was investigated. Based on single-factor tests, Box-Benhnken center composite experiment was carried out with three factors and three levels of experimental design using extraction time, extraction temperature and p H as the influence factors. Besides, the content of flavonoids was determined by Al (NO3) 3-NaNO2-NaOH spectrophotometric method. For a stable ionic liquid aqueous two-phase system, the phase-forming salt is ammonium sulfate ((NH4) 2SO4); the addition amount is 0. 8 g; and the volume ratio of the [Bmim]BF4 to water was 2: 2. Response surface analysis showed that for 0. 1 g of camphor leaves powder, the influence of factors that affected the extraction rate of flavonoids were in a descending order of extraction time, extraction temperature and pH. The optimized extraction conditions were as follows: extraction time 3. 7 h, extraction temperature 82℃, and the solution acidity 9. 6. Under the optimum conditions, the extraction rate of flavonoids reached 10. 62％. At the same time, the secondary mathematical model for extraction of total flavonoids from camphor leaves with ionic liquid aqueous two-phase system was established, which has a good predictive effect on target product extraction, and provided theoretical guidance for the scale-up experiment or large-scale production of this system.关键词
离子液体双水相/黄酮/响应面Key words
ionic liquid-aqueous two-phase system/flavonoids/response surface methodology引用本文复制引用
刘宝亮,曹桂萍..响应面分析法优化离子液体双水相提取香樟叶中总黄酮的工艺条件[J].现代食品科技,2018,34(4):179-187,9.基金项目
国家自然科学基金青年基金项目(51704042) （51704042）
常州工学院科研基金项目(YN1210) （YN1210）
