现代食品科技2018，Vol.34Issue(4)：221-226,6.DOI:10.13982/j.mfst.1673-9078.2018.04.033
超高效液相色谱串联质谱检测动物组织中卡马西平及其代谢物的残留量
Simultaneous Determination of Carbamazepine and Its Metabolites in Animal Matrix Using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
摘要
Abstract
Carbamazepine is a drug for the treatment of psychiatric illnesses, such as epilepsy, trigeminal neuralgia and so on. However, it also can stimulate the release of antidiuretic hormone, causing side effect of water-sodium retention. Water-sodium retention can lead to tissue edema and weight disorders, which provides the possibility of water retention and antidiuretic by illegal water injection in the breeding process. Therefore, it is of great significance to detect carbamazepine in animal tissues for cracking down on illegal water injection. A multiresidue method based on ultra performance liquid chromatography tandem mass spectrometry was developed for the determination of carbamazepine and its metabolites in animal matrix. In this method, samples were extracted by acidified acetonitrile, and purified by Oasis PRi ME HLB SPE column, and then carbamazepine and its metabolites were analyzed by Acquity UPLC BEH C18 column (100 mm×2. 1 mm, 1. 7 μm) using methanol and 0. 1％ formic acid as the mobile phases. The signals were acquired through the MRM mode. All analytes could be well-separated by a gradient program during 8 minutes. The calibration curves of carbamazepine and its metabolites had a good linear in a concentration range of 1. 050. 0 μg/L with the correlation coefficient more than 0. 999, and the limits of quantitation were all less than 1. 0 μg/kg in animal matrix. The recoveries of 1. 0, 10, 20 μg/kg fortified samples ranged from 72. 3％ to 106. 8％, with RSDs of 1. 43％ to 10. 3％. The new method provides a certain reference for the prevention of illicit drug addition and water injection in animal products.关键词
抗利尿激素/卡马西平/代谢物/超高效液相色谱串联质谱/注水肉/水钠潴留Key words
antidiuretic hormone/carbamazepine/metabolites/UPLC-MS/MS/affusion meat/water-sodium retention引用本文复制引用
杨盛茹,姚喜梅,邹建,刘洪斌..超高效液相色谱串联质谱检测动物组织中卡马西平及其代谢物的残留量[J].现代食品科技,2018,34(4):221-226,6.基金项目
十二五国家科技支撑计划项目(2014BAD13B05) （2014BAD13B05）
河南省重点科技攻关项目(162102210107) （162102210107）
