现代食品科技2018，Vol.34Issue(7)：28-34,7.DOI:10.13982/j.mfst.1673-9078.2018.7.005
木瓜提取物对小鼠脂肪肝的保护作用
Protective Effects of Active Components Extracted from Chaenomeles Speciosa on Non-alcoholic Fatty Liver Disease
摘要
Abstract
The protective effects of active components extracted from Chaenomeles Speciosa (EACCS) on non-alcoholic fatty liver disease (NAFLD) in mice were investigated. Forty male KM mice were equally randomized into four groups, namely, normal group, model group, low-dose EACCS group (100 mg/kg body weight/day), and high-dose EACCS group (300 mg/kg body weight/day). The normal group was fed with a normal diet, the model group was given a high-fat-high-fructose diet (HFFD), and the low-dose and high-dose EACCS groups were given HFFD followed by EACCS at low-and high-dose, respectively. Body weights were monitored. The mice were sacrificed 1 month later and serum-based biomarkers and morphology of liver tissue were examined. Real-time (RT)-PCR and confocal microscopy techniques were used to detect the expression of lipid metabolism-related genes. Compared with the normal group, the serum alanine aminotransferase (ALT) and total cholesterol (TC) of the model group increased, indicating significant steatosis; The expression levels of hepatocyte growth factor (HGF), vascular endothelial growth factor-A (VEGF-A) and c-Met also decreased in the model group, whilst the expression level of mi R-199 a-5 p increased. Compared with the model group, the hepatic lipid accumulation of EACCS groups was obviously suppressed with significantly decreased serum ALT and TC levels and the expression levels of hepatic HGF, VEGFa and c-Met m RNA increased; and the expression levels of mi R-199 a-5 p increased. Therefore, EACCS has protective effects on NAFLD mice induced by HFFD, and its mechanism may be related to the activation of mi R-199 a-5 p-HGF/c-Met signaling pathway in the liver tissues.关键词
木瓜提取物/非酒精性脂肪性肝病/HGF/c-Met/miR-199a-5pKey words
extracted active components of Chaenomeles Speciosa/non-alcoholic fatty liver disease/HGF/c-Met/mi R-199a-5p引用本文复制引用
李聪,熊海容,彭晓蔓,魏承亮,段丽,佘慧宇,张长城,袁丁,刘朝奇..木瓜提取物对小鼠脂肪肝的保护作用[J].现代食品科技,2018,34(7):28-34,7.基金项目
国家自然科学基金项目 (81473461) （81473461）
