现代食品科技2018，Vol.34Issue(7)：42-49,7,9.DOI:10.13982/j.mfst.1673-9078.2018.7.007
一种羧甲基茯苓多糖的结构及生物活性
Structure and Bioactivities of a Carboxymethyl Polysacchairde from Pachyman
摘要
Abstract
A carboxymethyl polysaccharide from Pachyman was isolated and purified using DEAE-52 cellulose column, Sephadex-G200 column and Sephadex-G150 column in the work. The structure of this polysacchairde was identified and its anti-proliferation activity and anti-inflammation activity investigated. This polysaccharide is a (1→3)-β-D-glucan with an average molar mass of 20.96×104 u, which also has a little (1→2) glucosidic bonds. Its monosaccharide composition only contained D-glucose and the triple helical structure did not exist. This polysacchairde exhibited a strong anti-proliferation effect on HT-29, Hep G-2, SGC-7901, MCF-7 and A549 cells, with IC50 values of 140.5 μg/m L, 264.3 μg/m L, 102.5 μg/m L, 256.4 μg/m L and 313.2 μg/m L, respectively. In addition, this polysaccharide exhibited an inhibitory effect on LPS-induced RAW264.7 cells, but could activate RAW264.7 cells in the absence of LPS. The findings would be of great importance for the development of Pachyman polysaccharide-based health foods and drugs.关键词
羧甲基茯苓多糖/结构/抗肿瘤活性/抗炎活性Key words
Carboxymethyl polysacchairde/structure/anti-proliferation activity/anti-inflammation activity引用本文复制引用
刘晓菲,胡双飞,张学武..一种羧甲基茯苓多糖的结构及生物活性[J].现代食品科技,2018,34(7):42-49,7,9.基金项目
广州市产学研协同创新重大专项 (201704020024) （201704020024）
