现代食品科技2018，Vol.34Issue(7)：50-55,6.DOI:10.13982/j.mfst.1673-9078.2018.7.008
猪源金黄色葡萄球菌lsa (E)基因的流行性研究
Characterization of the Prevalence and Distribution of Lsa (E) gene in Staphylococcus aureus Isolated from Pig Farms
摘要
Abstract
The spread of foodborne multi-resistant bacteria poses a serious threat to food safety and public health. The lsa (E) gene confers resistance to lincosamides, pleuromutilinsand streptogramin A. In order to investigate the prevalence and distribution oflsa (E) gene and multi-resistance gene clusters in Staphylococcus aureusisolated from pig farms in Xiamen. The lsa (E) gene and the genetic environment were tested by polymerase chain reaction (PCR) method. Then, lsa (E) positive S. aureus were characterized by drug susceptibility testing. In total, gene cluster: aad E-spc-lsa (E)-lnu (B)-tnp was found in 28 out of 29 lsa (E) positive S. aureus. Resistance was most frequently observed to clindamycin (100.0％), clarithromycin (100.0％) followed by trimethoprim/sulfamethoxazole (96.6％), tetracycline (96.6％), ciprofloxacin (96.6％), and gentamicin (72.4％). All the isolates were susceptible to oxacillin, quinupristin-dalfopristin, and linezolid. The multidrug resistance rate was 100.0％, and the main resistance profiles of S. aureus strains was PEN-GEN-TET-CLA-SXT-CLI-CIP-MXF. This study provides a theoretical basis for the prevention and treatment the food quality and safety problem caused by foodborne pathogenic bacteria and the controlling of multi-resistant strains spread of S. aureus in the food chain.关键词
金黄色葡萄球菌/耐药基因/多重耐药/基因簇Key words
Staphylococcus aureus/resistance genes/multi-resistance/gene clusters引用本文复制引用
谷立慧,周文渊,王丽,闫鹤..猪源金黄色葡萄球菌lsa (E)基因的流行性研究[J].现代食品科技,2018,34(7):50-55,6.基金项目
"十三五"国家重点研发计划 (2016YFD0500606) （2016YFD0500606）
广东省科技计划项目 (2014A020214001 （）
2016A020219001) （）
中央高校基本科研业务费 (D2170320) （D2170320）
中央高校建设世界一流大学 (学科) 和特色发展引导专项资金 (K5174960) （学科）
