现代食品科技2018，Vol.34Issue(7)：224-229,6.DOI:10.13982/j.mfst.1673-9078.2018.7.033
分子印迹固相萃取-高效液相色谱法检测鸡肉中的四环素类抗生素残留
Determination of Tetracycline Antibiotic Residues in Chicken by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography
摘要
Abstract
A selective molecularly imprinted polymer for separating tetracycline antibiotics was synthesized via bulk polymerization using tetracycline hydrochloride as the template, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and azobisisobutyronitrile as an initiator. This synthesized material was used as the solid phase adsorbent that was coupled with high performance liquid chromatography (HPLC) to establish a solid phase extraction-HPLC system for the determination of trace residues of three tetracycline antibiotics in chicken meat. The obtained results showed that the material has high adsorption capacity and specific recognition. A good linearity of the three tetracycline antibiotics in the range of 0.1-1.0 mg/L (R2> 0.99) was detected, with the minimum detection limits of oxytetracycline, tetracycline, doxycycline as 0.13 μg/L, 0.12 μg/L, 0.14 μg/L, respectively, and relative standard deviation (RSD) values of the five replicates as 1.27％, 1.31％, 1.94％, respectively. Whentetracycline antibiotics were added to chicken at three different concentrations (50 μg/kg, 100 μg/kg and 200 μg/kg, respectively), the recoveries were 88.33％～94.95％, 80.94％～88.02％, and 87.69％～93.08％, respectively, with corresponding RSD values being 2.08％～3.63％, 1.46％～2.37％, and 1.04％～3.03％, respectively. The results of this study show that the currently established method can be used for the detection of tetracycline residues in chicken meat.关键词
分子印迹聚合物/高效液相色谱/固相萃取/四环素Key words
molecularly imprinted polymers/high performance liquid chromatography/solid phase extraction/tetracycline引用本文复制引用
梁金玲,黄玉霞,何金兴..分子印迹固相萃取-高效液相色谱法检测鸡肉中的四环素类抗生素残留[J].现代食品科技,2018,34(7):224-229,6.基金项目
国家自然科学基金项目 (31301470) （31301470）
山东省优秀中青年科学家基金项目 (BS2012SW018) （BS2012SW018）
