中国食品学报2018，Vol.18Issue(8)：8-15,8.DOI:10.16429/j.1009-7848.2018.08.002
甜叶菊废渣提取物的抗氧化和抗炎作用
Antioxidant and Anti-inflammatory Activity of Stevia rebaudiana' Waste Extract
摘要
Abstract
Objective: The objective of the study was to evaluate the antioxidant and anti-inflammatory activity of Stevia rebaudiana waste extract. Methods: Two different extracts (SWE-1 and SWE-2) were obtained from Stevia rebaudiana waste by extraction and isolation. The antioxidant activity of Stevia rebaudiana waste extract was evaluated by DPPH, ABTS, FRAP, ORAC, and diet induced mice oxidative stress assays. The anti-inflammatory activity of Stevia rebaudiana waste extract was evaluated by lipopolysaccharide-induced murine macrophage inflammatory, dimethylbenzene induced ear swelling, and carrageenan induced paw edema assays. Results: Stevia rebaudiana waste extract could scavenge a variety of free radicals, showing strong antioxidant activity in vitro. SWE-1 significantly increased the activity of serum T-AOC, SOD and decreased the content of MDA in oxidative stress mice. Stevia rebaudiana waste extract significantly inhibited NO production in LPS induced macrophages, showing good anti-inflammatory activities. SWE-1 could inhibit dimethylbenzene induced ear swelling and carrageenan induced paw edema in mice, also decrease the inflammatory factor content of NO. Conclusion: Stevia rebaudiana waste extract had strong antioxidant and anti-inflammatory activities in vitro and in vivo. With a good prospect of development and utilization, it could be used as a functional food or nutraceutical product.关键词
甜叶菊/抗氧化/抗炎/氧化应激/巨噬细胞Key words
Stevia rebaudiana/antioxidant/anti-inflammatory/oxidative stress/macrophage引用本文复制引用
赵磊,迟茜,林文轩,王成涛,徐美利,连运河,杨香瑜,李乾丽..甜叶菊废渣提取物的抗氧化和抗炎作用[J].中国食品学报,2018,18(8):8-15,8.基金项目
"十三五"北京市属高校高水平教师队伍建设支持计划 (CIT&TCD201704042) （CIT&TCD201704042）
北京市科技计划项目 (Z171100002217019) （Z171100002217019）
国家重点研发计划项目 (2016YFD0400802) （2016YFD0400802）
北京市属高校创新能力提升计划项目 (0142132016) （0142132016）
