中国食品学报2018，Vol.18Issue(8)：107-114,8.DOI:10.16429/j.1009-7848.2018.08.015
耐高温、高醇醋酸菌筛选、鉴定及低能离子选育
Screening, Identification of a Thermoresistant and Alcohol-tolerant Acetic Acid Bacterium and Breeding of Imbecile Ion Beam
摘要
Abstract
In this study, Acetobacter was firstly screened from the decayed kiwifruit, which were cultured in liquid medium with different concentrations of ethanol at different temperatures, WT08 stain with the highest acetic acid production at the conditions of high temperature and high alcohol was screened, whose acetic acid production reached 39.09 g/L when cultured in the medium with 6％ ethanol at 32 ℃ for 72 h, 12.99 g/L when cultured with 12％ ethanol, and5.89 g/L when cultured in the minimum medium at 40 ℃ for 72 h. It was initially characterized as Acetobacter pasteurianus by testing its morphology, physiology, biochemistry and 16 srDNA nucleotide sequence. WT08-18 was obtained from the strain WT08 by N+ (10 keV, 70 ×2.6 ×1013 ions/cm2) implantation and heredity of the strain was stable. The yield of total acid was 49.86 g/L, the yield of acid was 15.16 g/L under 12％ ethanol and the yield of acid was 10.96 g/L quiescently cultured 72 h at 40 ℃ by WT08-18. The liquid state seed of WT08-18 was inoculated into the solid fermentation substrate of Hengshun Wantong aromatic vinegar. The results showed that the yield of acetic acid and the conversion rate of alcohol to acetic acid had increased by 30.54％ and 18.81％ respectively. It had potential application value in the industrial production.关键词
醋酸菌/低能离子/修饰/耐高温/耐高乙醇Key words
Acetobacter/low-energy ion beam/modification/thermoresistant/alcohol-tolerant引用本文复制引用
李文,王陶,谢昊讯,高明侠,董玉玮,李同祥,张传丽..耐高温、高醇醋酸菌筛选、鉴定及低能离子选育[J].中国食品学报,2018,18(8):107-114,8.基金项目
江苏省重点研发计划项目 (BE2016648) （BE2016648）
江苏省高校自然科学研究重大项目 (16KJA210001) （16KJA210001）
江苏省"333"工程科研项目 (BRA2016274) （BRA2016274）
