中国食品学报2018，Vol.18Issue(8)：254-259,6.DOI:10.16429/j.1009-7848.2018.08.033
高效液相色谱法检测乳中β-内酰胺酶抑制剂——克拉维酸
Determination of β-Lactamase Inhibitor——Clavulanic Acid in Milk by High Performance Liquid Chromatography
摘要
Abstract
An analytical method was developed for the determination of β-lactamase inhibitor clavulanic acid in milk by high performance liquid chromatography. The sample was deproteinized by acetonitrile and degreased by n-hexane.The chromatographic conditions was chosen Hypersil ODS-2 C18 (4.6 mm×250 mm, 5 μm) chromatographic column using0.025 mol/L of potassium dihydrogen phosphate (pH 4.5)-methanol (95 ∶ 5, V/V) as mobile phases, detection wavelength was217 nm and velocity was 1 mL/min. The results that the calibration curve showed perfect linear in the range of 5～200 μg/mL and the correlation coefficient is 0.9992. Matrix-matched calibration standard was used for the quantification. The average recoveries ranged from 83％ to 92％ with relative standard deviation less than 1.185％. With the advantages of convenience, sensitivity, reliable and accurate results, the method was suitable for the detection of β-lactamase inhibitor clavulanic acid in milk.关键词
高效液相色谱/β-内酰胺酶抑制/克拉维酸/牛乳Key words
high performance liquid chromatography/β-lactamase inhibitor/clavulanic acid/milk引用本文复制引用
郭佩佩,姚宇秀,王艳菲,陈超,于国萍..高效液相色谱法检测乳中β-内酰胺酶抑制剂——克拉维酸[J].中国食品学报,2018,18(8):254-259,6.基金项目
国家科技支撑计划课题 (2013BAD18B00) （2013BAD18B00）
