食品科学2018，Vol.39Issue(24)：93-101,9.DOI:10.7506/spkx1002-6630-201824015
抗食源性致病菌益生菌的筛选及其抑菌活性评价
Screening and Evaluation of Probiotics for Antimicrobial Activity against Foodborne Pathogenic Bacteria
摘要
Abstract
Cross streak,radial streak,agar diffusion and co-culture methods were used to assess the antimicrobial activity of various probiotics and their metabolites against four representative foodborne pathogenic bacteria.The probiotic strains with great antibacterial activity against both Gram-positive pathogens(Listeria monocytogenes and Staphylococcus aureus)and Gram-negative pathogens(Escherichia coli and Salmonella typhimurium)were screened out.They were incorporated into polymeric materials(hydroxypropyl cellulose,glycerol and konjac flour)to develop bacteriostatic films,and the probiotic viability and the antimicrobial effects of films were measured.The consistent results obtained from cross streak and radial streak tests showed that Lactobacillus and Enterococcus especially Lactobacillus paracasei CICC 20241 exhibited high antibacterial activity against four target pathogens;the agar diffusion test showed that the lactic acid bacteria culture(LC),cell-free supernatant(CFS),and bacterial cells(BC)of L.paracasei CICC 20241 all had great antibacterial effect,and the co-culture test showed that L.paracasei CICC 20241 exhibited higher growth activity and antagonistic activity when inoculated earlier than the pathogens.In addition,the bacteriostatic film prepared in this experiment was a good carrier for L.paracasei CICC 20241,which was suitable for the survival of the Lactobacillus strain and exhibited continuous antibacterial activity against the target pathogens.The Lactobacillus film may have a great application potential for the development of packaging materials for fresh foods.关键词
益生菌/抑菌活性/致病菌/生物活性复合膜Key words
probiotics/antimicrobial activity/pathogens/bioactive composite film分类
轻工纺织引用本文复制引用
戴璐,袁亚宏,张宇翔,魏建平,岳田利..抗食源性致病菌益生菌的筛选及其抑菌活性评价[J].食品科学,2018,39(24):93-101,9.基金项目
国家自然科学基金面上项目(31371814 （）
31671866) （）
国家重点基础研究发展计划(973计划)项目(2013FY113400) （973计划）
