食品科学技术学报2019，Vol.37Issue(1)：28-35,8.DOI:10.3969/j.issn.2095-6002.2019.01.005
黄曲霉产胞外β-1 ,3-1 ,4-葡聚糖酶的发酵条件优化
Optimization of Fermentation Conditions for β-1,3-1,4-Glucanase Production by Aspergillus flavus
摘要
Abstract
An Aspergillus flavus with the abilit≥to≥ield high production of β-1,3-1,4-glucanase was found. The fermentation conditions for this enz≥me in the shake flask were optimized using a combination of one-single-factor method and response surface methodolog≥. The results indicated that the optimal culture medium components for β-1,3-1,4-glucanase production were 19 g/L wheat bran, 30 g/L ( NH4 )2 HPO4 , 21 g/L Tween-60, 5 g/L NaCl, 0. 5 g/L MgSO4·7H2 O and 0. 75 g/L KH2 PO4 at an initial pH of 8. 0. The high-est enz≥me activit≥of 155. 9 U/mL was obtained when the strain was cultured at 38℃ for 6 d in the opti-mized culture medium. The crude β-1,3-1,4-glucanase from Aspergillus flavus could h≥drol≥ze oat and barle≥ powder to mainl≥≥ield glucose. These results indicated that the extracellular β-1,3-1,4-glucanase produced b≥ Aspergillus flavus possess great potential for its industrial applications.关键词
黄曲霉/ β-1, 3-1, 4-葡聚糖酶/发酵条件/响应面分析/水解Key words
Aspergillus flavus/β-1, 3-1, 4-glucanase/fermentation condition/response surface methodolog≥/h≥drol≥sis分类
轻工纺织引用本文复制引用
刘璐,陈洲,陈瑶瑶,刘杨柳,李思霆,贾英民..黄曲霉产胞外β-1 ,3-1 ,4-葡聚糖酶的发酵条件优化[J].食品科学技术学报,2019,37(1):28-35,8.基金项目
国家自然科学基金资助项目(31771907) . （31771907）
